{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T12:30:55Z","timestamp":1775305855349,"version":"3.50.1"},"reference-count":51,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,1,1]]},"abstract":"<jats:p>CVD (cardiovascular disease) is associated with abnormal liver enzymes, and NAFLD (non-alcoholic fatty liver disease) is independently associated with cardiovascular risk. To gain insights into the molecular events underlying the association between liver enzymes and CVD, we developed an HFD (high-fat diet)-induced NAFLD in the SHR (spontaneously hypertensive rat) and its control WKY (Wistar\u2013Kyoto) rat strain. We hypothesized that hepatic induction of Hif1a (hypoxia-inducible factor 1\u03b1) might be the link between CVD and liver injury. Male SHRs (n=13) and WKY rats (n=14) at 16\u00a0weeks of age were divided into two experimental groups: standard chow diet and HFD (10\u00a0weeks). HFD-fed rats, irrespective of the strain, developed NAFLD; however, only HFD-SHRs had focus of lobular inflammation and high levels of hepatic TNF\u03b1 (tumour necrosis factor \u03b1). SHRs had significantly higher liver weight and ALT (alanine aminotransferase) levels, irrespective of NAFLD. Liver abundance of Hif1a mRNA and Hif1\u03b1 protein were overexpressed in SHRs (P&amp;lt;0.04) and were significantly correlated with ALT levels (R=0.50, P&amp;lt;0.006). This effect was not reverted by a direct acting splanchnic vasodilator (hydralazine). Angiogenesis may be induced by the HFD, but the disease model showed significantly higher hepatic Vegf (vascular endothelial growth factor) levels (P&amp;lt;0.025) even in absence of dietary insult. Hif1a mRNA overexpression was not observed in other tissues. Liver mRNA of Nr1d1 (nuclear receptor subfamily 1, group D, member 1; P&amp;lt;0.04), Ppara [Ppar (peroxisome-proliferatoractivated receptor) \u03b1; P&amp;lt;0.05], Pparg (Ppar\u03b3; P&amp;lt;0.001) and Sirt1 (Sirtuin 1; P&amp;lt;0.001) were significantly upregulated in SHRs, irrespective of NAFLD. Sirt1 and Hif1a mRNAs were significantly correlated (R=0.71, P&amp;lt;0.00002). In conclusion, CVD is associated with Hif1a-related liver damage, hepatomegaly and reprogramming of liver metabolism, probably to compensate metabolic demands.<\/jats:p>","DOI":"10.1042\/cs20120151","type":"journal-article","created":{"date-parts":[[2012,7,27]],"date-time":"2012-07-27T23:14:55Z","timestamp":1343430895000},"page":"53-63","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Cardiovascular disease is associated with high-fat-diet-induced liver damage and up-regulation of the hepatic expression of hypoxia-inducible factor 1\u03b1 in a rat model"],"prefix":"10.1042","volume":"124","author":[{"given":"Adriana\u00a0L.","family":"Burgue\u00f1o","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Biology of Complex Diseases, Institute of Medical Research A Lanari-IDIM, University of Buenos Aires\u2013National Council of Scientific and Technological Research (CONICET), Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas\u00a0F.","family":"Gianotti","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Biology of Complex Diseases, Institute of Medical Research A Lanari-IDIM, University of Buenos Aires\u2013National Council of Scientific and Technological Research (CONICET), Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noelia\u00a0G.","family":"Mansilla","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Biology of Complex Diseases, Institute of Medical Research A Lanari-IDIM, University of Buenos Aires\u2013National Council of Scientific and Technological Research (CONICET), Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos\u00a0J.","family":"Pirola","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Biology of Complex Diseases, Institute of Medical Research A Lanari-IDIM, University of Buenos Aires\u2013National Council of Scientific and Technological Research (CONICET), Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Sookoian","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Hepatology, Institute of Medical Research A Lanari-IDIM, University of Buenos Aires\u2013National Council of Scientific and Technological Research (CONICET), Ciudad Aut\u00f3noma de Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2012,9,7]]},"reference":[{"key":"2021113010254464900_B1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1161\/ATVBAHA.107.147538","article-title":"Fatty liver: a novel component of the metabolic syndrome","volume":"28","author":"Kotronen","year":"2008","journal-title":"Arterioscler., Thromb., Vasc. 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