{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T21:09:39Z","timestamp":1790024979332,"version":"4.0.1"},"reference-count":41,"publisher":"Portland Press Ltd.","issue":"6","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,9,1]]},"abstract":"<jats:p>DTH (delayed type\u00a0hypersensitivity) reactions are secondary cellular immune responses that appear 24\u201372\u00a0h after antigen exposure. Tuberculous pleurisy is a common manifestation of extrapulmonary TB (tuberculosis) and is considered a human model of Th1-mediated DTH. In order to identify functional cross-talk among cellular populations sited in this inflammatory microenvironment, we analysed phenotypic and functional features of human B-cells isolated from the PF (pleural fluid) of TB patients. Freshly isolated PF-B-cells displayed a lower expression of CD20, CD1d and HLA-DR, and a higher expression of CD95, CD38, CD25, CXCR3 (CXC chemokine receptor 3) and CXCR4 (CXC chemokine receptor 4) than their PB (peripheral blood) counterparts, suggesting a non-classical in situ activation. Although memory PF-T-cell frequencies were increased, the frequencies of memory PF-B-cells were not. We demonstrated that, upon stimulation with \u03b3-irradiated M. tuberculosis, mycobacterially secreted proteins or a lectin mitogen, PF-B-cells had a strong activation and produced IL-10 by a mechanism that was dependent on bystander activation of CD19\u2212 PF cells. Besides, within PF cells, B-cells diminished in\u00a0vitro M. tuberculosis-induced IFN (interferon)-\u03b3 production by T-cells and NK (natural killer) cells in an IL-10-dependent manner. Finally, we found that the lower the frequency of B-cells, the higher the ratio of IFN-\u03b3\/IL-10 within PF. Thus our results suggest that B-cells can regulate a human DTH reaction induced by M. tuberculosis.<\/jats:p>","DOI":"10.1042\/cs20130769","type":"journal-article","created":{"date-parts":[[2014,4,2]],"date-time":"2014-04-02T08:31:11Z","timestamp":1396427471000},"page":"391-403","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Human pleural B-cells regulate IFN-\u03b3 production by local T-cells and NK cells in a<i>Mycobacterium tuberculosis<\/i>-induced delayed hypersensitivity reaction"],"prefix":"10.1042","volume":"127","author":[{"given":"Pablo","family":"Schierloh","sequence":"first","affiliation":[{"name":"Inmunologia de Enfermedades Respiratorias, Instituto de Medicina Experimental (IMEX)-CONICET, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ver\u00f3nica","family":"Landoni","sequence":"additional","affiliation":[{"name":"Inmunologia de Enfermedades Respiratorias, Instituto de Medicina Experimental (IMEX)-CONICET, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana","family":"Balboa","sequence":"additional","affiliation":[{"name":"Inmunologia de Enfermedades Respiratorias, Instituto de Medicina Experimental (IMEX)-CONICET, Academia Nacional de Medicina, Pacheco de Melo 3081, 1425, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa\u00a0M.","family":"Musella","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Neumonolog\u00eda, Hospital F.J Mu\u00f1iz, Uspallata 2272, 1282, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Castagnino","sequence":"additional","affiliation":[{"name":"Divisi\u00f3n de Neumonolog\u00eda, Hospital F.J Mu\u00f1iz, Uspallata 2272, 1282, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Mora\u00f1a","sequence":"additional","affiliation":[{"name":"Instituto de Tisioneumonologia Dr R. 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