{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:04:17Z","timestamp":1790643857271,"version":"4.1.0"},"reference-count":39,"publisher":"Portland Press Ltd.","issue":"11","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:p>Habitual aerobic exercise prevents age-related impairments in endothelium-dependent dilation (EDD). We have hypothesized that the pro-inflammatory transcription factor nuclear factor \u03baB (NF-\u03baB) impairs EDD with sedentary aging, and habitual aerobic exercise prevents this age-related suppression of EDD by NF-\u03baB. To test this hypothesis, we have inhibited NF-\u03baB signalling via oral salsalate administration in healthy older aerobic exercise-trained adults (OT, n=14, 58\u00b12\u00a0years), older non-exercising adults (ON, n=16, 61\u00b11\u00a0years) and young non-exercising controls (YN, n=8, 23\u00b11\u00a0years). Salsalate reduced endothelial cell expression of NF-\u03baB p65 by ~25% in ON (P&amp;lt;0.05) but did not significantly change expression in OT or YN (P&amp;gt;0.05). EDD, assessed by brachial artery flow-mediated dilation (FMD), was improved by salsalate in ON (4.0\u00b10.7% compared with 6.8\u00b10.7%, placebo compared with salsalate, P&amp;lt;0.001) but did not change with salsalate in OT or YN (OT: 7.2\u00b10.7% compared with 7.7\u00b10.6%; YN: 7.6\u00b10.9% compared with 8.1\u00b10.8%; placebo compared with salsalate, P&amp;gt;0.05). Endothelium-independent dilation was not affected by salsalate in any group (P&amp;gt;0.05). In ON, vitamin C infusion improved FMD by ~30% during placebo (P&amp;lt;0.001) but had no affect during salsalate (P&amp;gt;0.05). In OT and YN, vitamin C infusion did not affect FMD during either placebo or salsalate (P&amp;gt;0.05). Salsalate reduced endothelial cell nitrotyrosine content by ~25% and NADPH oxidase p47phox expression by ~30% in ON (P&amp;lt;0.05) but had no effect in OT or YN (P&amp;gt;0.05). Our results suggest that endothelial NF-\u03baB signalling is associated with oxidative stress-related impairment of EDD in healthy non-exercising but not aerobically exercising older adults. This may be a key mechanism by which regular aerobic exercise preserves endothelial function and reduces cardiovascular risk with aging.<\/jats:p>","DOI":"10.1042\/cs20140030","type":"journal-article","created":{"date-parts":[[2014,6,23]],"date-time":"2014-06-23T04:38:11Z","timestamp":1403498291000},"page":"645-654","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["Prevention of age-related endothelial dysfunction by habitual aerobic exercise in healthy humans: possible role of nuclear factor \u03baB"],"prefix":"10.1042","volume":"127","author":[{"given":"Ashley\u00a0E.","family":"Walker","sequence":"first","affiliation":[{"name":"Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachelle\u00a0E.","family":"Kaplon","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary\u00a0L.","family":"Pierce","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly\u00a0J.","family":"Nowlan","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas\u00a0R.","family":"Seals","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2014,7,29]]},"reference":[{"key":"2021113010092933400_B1","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1016\/j.jacc.2007.11.044","article-title":"Prognostic role of flow-mediated dilation and cardiac risk factors in post-menopausal women","volume":"51","author":"Rossi","year":"2008","journal-title":"J. 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