{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T08:28:26Z","timestamp":1790497706353,"version":"4.1.0"},"reference-count":40,"publisher":"Portland Press Ltd.","issue":"9","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,11,1]]},"abstract":"<jats:p>Liver monocytes play a major role in the development of NASH (non-alcoholic steatohepatitis). In inflamed tissues, monocytes can differentiate in both macrophages and dendritic cells. In the present study, we investigated the role of moDCs (monocyte-derived inflammatory dendritic cells) in experimental steatohepatitis induced in C57BL\/6 mice by feeding on a MCD (methionine\/choline-deficient) diet. The evolution of steatohepatitis was characterized by an increase in hepatic CD45+\/CD11b+ myeloid cells displaying the monocyte\/macrophage marker F4-80+. In the early phases (4\u00a0weeks of treatment), Ly6Chigh\/CD11b+\/F4-80+ inflammatory macrophages predominated. However, their frequency did not grow further with the disease progression (8\u00a0weeks of treatment), when a 4-fold expansion of CD11b+\/F4-80+ cells featuring the fractalkine receptor (CX3CR1) was evident. These CX3CR1+ cells were also characterized by the combined expression of inflammatory monocyte (Ly6C, CD11b) and dendritic cell (CD11c, MHCII) markers as well as by a sustained TNF\u03b1 (tumour necrosis factor \u03b1) production, suggesting monocyte differentiation into inflammatory moDCs. The expansion of TNF\u03b1-producing CX3CR1+ moDCs was associated with an elevation in hepatic and circulating TNF\u03b1 level and with the worsening of parenchymal injury. Hydrogen sulfide (H2S) has been shown to interfere with CX3CR1 up-regulation in monocyte-derived cells exposed to pro-inflammatory stimuli. Treating 4-week-MCD-fed mice with the H2S donor NaHS while continuing on the same diet prevented the accumulation of TNF\u03b1-producing CX3CR1+ moDCs without interfering with hepatic macrophage functions. Furthermore, NaHS reduced hepatic and circulating TNF\u03b1 levels and ameliorated transaminase release and parenchymal injury. Altogether, these results show that inflammatory CX3CR1+ moDCs contributed in sustaining inflammation and liver injury during steatohepatitis progression.<\/jats:p>","DOI":"10.1042\/cs20150053","type":"journal-article","created":{"date-parts":[[2015,8,7]],"date-time":"2015-08-07T11:54:51Z","timestamp":1438948491000},"page":"797-808","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":80,"title":["CX3CR1-expressing inflammatory dendritic cells contribute to the progression of steatohepatitis"],"prefix":"10.1042","volume":"129","author":[{"given":"Salvatore","family":"Sutti","sequence":"first","affiliation":[{"name":"Department of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University \u201cAmedeo Avogadro\u201d of East Piedmont, Via Solaroli 17, 28100 Novara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Locatelli","sequence":"additional","affiliation":[{"name":"Department of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University \u201cAmedeo Avogadro\u201d of East Piedmont, Via Solaroli 17, 28100 Novara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Bruzz\u00ec","sequence":"additional","affiliation":[{"name":"Department of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University \u201cAmedeo Avogadro\u201d of East Piedmont, Via Solaroli 17, 28100 Novara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aastha","family":"Jindal","sequence":"additional","affiliation":[{"name":"Department of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University \u201cAmedeo Avogadro\u201d of East Piedmont, Via Solaroli 17, 28100 Novara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Vacchiano","sequence":"additional","affiliation":[{"name":"Department of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University \u201cAmedeo Avogadro\u201d of East Piedmont, Via Solaroli 17, 28100 Novara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Bozzola","sequence":"additional","affiliation":[{"name":"Department of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University \u201cAmedeo Avogadro\u201d of East Piedmont, Via Solaroli 17, 28100 Novara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Albano","sequence":"additional","affiliation":[{"name":"Department of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University \u201cAmedeo Avogadro\u201d of East Piedmont, Via Solaroli 17, 28100 Novara, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2015,8,7]]},"reference":[{"key":"2021113010391608900_B1","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1055\/s-0028-1091978","article-title":"The epidemiology of nonalcoholic fatty liver disease: a global perspective","volume":"28","author":"Lazo","year":"2008","journal-title":"Semin. 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