{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:33:17Z","timestamp":1773300797012,"version":"3.50.1"},"reference-count":51,"publisher":"Portland Press Ltd.","issue":"9","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,5,1]]},"abstract":"<jats:p>Corneal neovascularization, the growth of new blood vessels in the cornea, is a leading cause of vision impairment after corneal injury. Neovascularization typically occurs in response to corneal injury such as that caused by infection, physical trauma, chemical burns or in the setting of corneal transplant rejection. The NADPH oxidase enzyme complex is involved in cell signalling for wound-healing angiogenesis, but its role in corneal neovascularization has not been studied. We have now analysed the role of the Nox2 isoform of NADPH oxidase in corneal neovascularization in mice following chemical injury. C57BL\/6 mice aged 8\u201314\u00a0weeks were cauterized with an applicator coated with 75% silver nitrate and 25% potassium nitrate for 8\u00a0s. Neovascularization extending radially from limbal vessels was observed in corneal whole-mounts from cauterized wild type\u00a0mice and CD31+ vessels were identified in cauterized corneal sections at day 7. In contrast, in Nox2 knockout (Nox2 KO) mice vascular endothelial growth factor-A (Vegf-A), Flt1 mRNA expression, and the extent of corneal neovascularization were all markedly reduced compared with their wild type\u00a0controls. The accumulation of Iba-1+ microglia and macrophages in the cornea was significantly less in Nox2 KO than in wild type\u00a0mice. In conclusion, we have demonstrated that Nox2 is implicated in the inflammatory and neovascular response to corneal chemical injury in mice and clearly VEGF is a mediator of this effect. This work raises the possibility that therapies targeting Nox2 may have potential for suppressing corneal neovascularization and inflammation in humans.<\/jats:p>","DOI":"10.1042\/cs20150103","type":"journal-article","created":{"date-parts":[[2016,1,27]],"date-time":"2016-01-27T01:18:32Z","timestamp":1453857512000},"page":"683-696","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["NADPH oxidase 2 plays a role in experimental corneal neovascularization"],"prefix":"10.1042","volume":"130","author":[{"given":"Elsa\u00a0C.","family":"Chan","sequence":"first","affiliation":[{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria 3002, Australia"},{"name":"Ophthalmology, Department of Surgery, University of Melbourne, East Melbourne, Victoria 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"van Wijngaarden","sequence":"additional","affiliation":[{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria 3002, Australia"},{"name":"Ophthalmology, Department of Surgery, University of Melbourne, East Melbourne, Victoria 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elsie","family":"Chan","sequence":"additional","affiliation":[{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria 3002, Australia"},{"name":"Ophthalmology, Department of Surgery, University of Melbourne, East Melbourne, Victoria 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darleen","family":"Ngo","sequence":"additional","affiliation":[{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiang-Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria 3002, Australia"},{"name":"Ophthalmology, Department of Surgery, University of Melbourne, East Melbourne, Victoria 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitesh\u00a0M.","family":"Peshavariya","sequence":"additional","affiliation":[{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory\u00a0J.","family":"Dusting","sequence":"additional","affiliation":[{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria 3002, Australia"},{"name":"Ophthalmology, Department of Surgery, University of Melbourne, East Melbourne, Victoria 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guei-Sheung","family":"Liu","sequence":"additional","affiliation":[{"name":"Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, East Melbourne, Victoria 3002, Australia"},{"name":"Ophthalmology, Department of Surgery, University of Melbourne, East Melbourne, Victoria 3002, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2016,3,18]]},"reference":[{"key":"2021113010475842600_B1","first-page":"264","article-title":"Corneal angiogenic privilege: angiogenic and antiangiogenic factors in corneal avascularity, vasculogenesis, and wound healing (an American Ophthalmological Society thesis)","volume":"104","author":"Azar","year":"2006","journal-title":"Trans. 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