{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T14:28:30Z","timestamp":1782743310719,"version":"3.54.5"},"reference-count":42,"publisher":"Portland Press Ltd.","issue":"14","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,7,1]]},"abstract":"<jats:p>miR-192-5p has gained increasing relevance in various diseases, however, its function in acute liver injury is currently unknown. We analysed miR-192-5p serum levels and hepatic miR-192-5p expression in mice after hepatic ischaemia and reperfusion (I\/R) as well as in toxic liver injury. On a functional level, miRNA levels were analysed in the different hepatic cell-compartments and in the context of tumour necrosis factor (TNF)-dependent liver cell death. We detected increased serum levels of miR-192-5p after hepatic I\/R- and carbon tetrachloride (CCl4)-induced liver injury. miR-192-5p levels correlated with the degree of liver damage and the presence of hepatic cell death detected by TUNEL stainings (terminal deoxynucleotidyltransferase-mediated dUTP biotin nick-end labelling stainings). Moreover, expression of miR-192-5p was increased in a hypoxia\/reoxygenation (H\/R) model of in\u00a0vitro hepatocyte injury, supporting that the passive release of miR-192-5p represents a surrogate for hepatocyte death in liver injury. In critically ill patients, miR-192-5p levels were elevated selectively in patients with liver injury and closely correlated with the presence of hepatic injury. In contrast with up-regulated miR-192-5p in the serum, we detected a down-regulation of miR-192-5p in both injured mouse and human livers. Deregulation of miR-192-5p in livers was dependent on stimulation with TNF. Functional experiments confirmed a protective effect of down-regulation of miR-192-5p in hepatocytes, suggesting a role of miR-192-5p in limiting liver injury. Finally, we identified Zeb2, an important regulator of cell death, as a potential target gene mediating the function of miR-192-5p. Our data suggest that miR-192-5p is involved in the regulation of liver cell death during acute liver injury and might represent a potent marker of hepatic injury.<\/jats:p>","DOI":"10.1042\/cs20160216","type":"journal-article","created":{"date-parts":[[2016,4,19]],"date-time":"2016-04-19T02:49:23Z","timestamp":1461034163000},"page":"1197-1207","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Down-regulation of <i>miR-192-5p<\/i> protects from oxidative stress-induced acute liver injury"],"prefix":"10.1042","volume":"130","author":[{"given":"Sanchari","family":"Roy","sequence":"first","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Benz","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Alder","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heike","family":"Bantel","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Hepatology and Endocrinology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joern","family":"Janssen","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihael","family":"Vucur","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremie","family":"Gautheron","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Sch\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Loosen","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Luedde","sequence":"additional","affiliation":[{"name":"Department of Cardiology and Angiology, University of Kiel, Arnold-Heller-Stra\u00dfe 3, 24105 Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Koch","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Tacke","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Luedde","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Trautwein","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Roderburg","sequence":"additional","affiliation":[{"name":"Department of Medicine III, University of Aachen (RWTH), Pauwelsstra\u00dfe 30, 52074 Aachen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2016,6,1]]},"reference":[{"key":"2021113010450006600_B1","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1016\/j.devcel.2006.09.009","article-title":"The diverse functions of microRNAs in animal development and disease","volume":"11","author":"Kloosterman","year":"2006","journal-title":"Dev. 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