{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:12:00Z","timestamp":1791274320805,"version":"4.1.0"},"reference-count":28,"publisher":"Portland Press Ltd.","issue":"23","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,12,1]]},"abstract":"<jats:p>Asthma is a chronic disease of the airways\u00a0affecting\u00a0one in ten children in Westernized countries.\u00a0Recently, our group showed that specific bacterial genera in early life are associated with atopy and wheezing in 1-year-old children. However, little is known about the link between the early life gut microbiome and the diagnosis of asthma in preschool age children. To determine the role of the gut microbiota in preschool age asthma, children up to 4\u00a0years of age enrolled in the Canadian Healthy Infant Longitudinal Development (CHILD) study were classified as asthmatic (n=39) or matched healthy controls (n=37). 16S rRNA sequencing and quantitative PCR (qPCR) were used to analyse the composition of the 3-month and 1-year gut microbiome of these children.\u00a0At 3 months the abundance of the genus,\u00a0Lachnospira\u00a0(L), was decreased (P=0.008), whereas the abundance of the species,\u00a0Clostridium neonatale\u00a0(C), was increased (P=0.07) in asthmatics. Quartile analysis of stool composition at 3-months revealed a negative association between the ratio of these two bacteria (L\/C) and asthma risk by 4 years of age [quartile 1: odds ratio (OR)=15, P=0.02, CI\u2009(confidence interval)=\u20091.8\u2013124.7; quartile 2: OR=1.0, ns; quartile 3: OR=0.37, ns]. We conclude that opposing shifts in the relative abundances of\u00a0Lachnospira\u00a0and\u00a0C. neonatale\u00a0in the first 3 months of life are associated with preschool age asthma, and that the L\/C ratio may serve as a potential early life biomarker to predict asthma development.<\/jats:p>","DOI":"10.1042\/cs20160349","type":"journal-article","created":{"date-parts":[[2016,9,15]],"date-time":"2016-09-15T21:48:59Z","timestamp":1473976139000},"page":"2199-2207","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":127,"title":["Shifts in <i>Lachnospira<\/i> and <i>Clostridium sp.<\/i> in the 3-month stool microbiome are associated with preschool age asthma"],"prefix":"10.1042","volume":"130","author":[{"given":"Leah\u00a0T.","family":"Stiemsma","sequence":"first","affiliation":[{"name":"Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada, V6T 1Z3"},{"name":"Child & Family Research Institute 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4H4"},{"name":"Department of Pediatrics, University of British Columbia, Vancouver, Canada, V6H 3V4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"the Canadian Healthy Infant Longitudinal Development (CHILD) Study Investigators","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William\u00a0W.","family":"Mohn","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada, V6T 1Z3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.\u00a0Brett","family":"Finlay","sequence":"additional","affiliation":[{"name":"Department of Microbiology & Immunology, University of British Columbia, Vancouver, Canada, V6T 1Z3"},{"name":"Michael Smith Laboratories, University of British Columbia, Vancouver, Canada, V6T 1Z4"},{"name":"Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada, V6T 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