{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:04:32Z","timestamp":1773799472826,"version":"3.50.1"},"reference-count":22,"publisher":"Portland Press Ltd.","issue":"16","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2018,8,31]]},"abstract":"<jats:p>There is a pressing need for new approaches to prevent stroke. Endothelial progenitor cells (EPCs) promote vascular repair and revascularization in the ischemic brain. The present study sought to evaluate whether preventive delivery of EPCs could prevent or protect against stroke. Stroke-prone spontaneously hypertensive rats (SHR-SP) received a single injection of EPCs, and their survival time was monitored. In addition, at 28 and\/or 42 days after a single injection of EPCs, SHR-SP and mice were subjected to cerebral ischemia, and cerebral ischemic injury, local angiogenesis and in vivo EPC integration were determined. Other experiments examined the effects of EPC conditioned medium, and the distribution of donor EPCs taken from GFP transgenic mice. It was found that EPC-pretreated SHR-SP showed longer lifespans than untreated controls. A single preventive injection of EPCs could produce persistent protective effects against cerebral ischemic injury (lasting at least 42 days), and promote local angiogenesis in the ischemic brain, in two types of animals (SHR-SP and normotensive mice). EPCs of donor origin could be detected in the recipient peripheral blood, and integrated into the recipient ischemic brains. Furthermore, it was suggested that mouse EPCs might exert paracrine effects on cerebral ischemic injury in addition to their direct angiogenic effects. In conclusion, a single preventive injection of EPCs prolonged the lifespan of SHR-SP, and protected against cerebral ischemic injury for at least 7 weeks. It is implied that EPC injection might be a promising candidate for a preventive role in patients at high risk for stroke.<\/jats:p>","DOI":"10.1042\/cs20180360","type":"journal-article","created":{"date-parts":[[2018,7,13]],"date-time":"2018-07-13T17:15:32Z","timestamp":1531502132000},"page":"1797-1810","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A preventive injection of endothelial progenitor cells prolongs lifespan in stroke-prone spontaneously hypertensive rats"],"prefix":"10.1042","volume":"132","author":[{"given":"Cheng","family":"Peng","sequence":"first","affiliation":[{"name":"School of Public Health and Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Hui","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia-Lin","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public Health and Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Long","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Fang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Identification of Traditional Chinese Medicine and Department of Pharmacology, The Second Military Medical University, Shanghai 200433, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Ping","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health and Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Long","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public Health and Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ding-Feng","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Identification of Traditional Chinese Medicine and Department of Pharmacology, The Second Military Medical University, Shanghai 200433, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200120, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Identification of Traditional Chinese Medicine and Department of Pharmacology, The Second Military Medical University, Shanghai 200433, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex\u00a0F.","family":"Chen","sequence":"additional","affiliation":[{"name":"Third Xiangya Hospital and the Institute of Vascular Disease and Translational Medicine, Central South University, Changsha, Hunan 410013, China"},{"name":"Institute of Development and Regenerative Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200092"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"He-Hui","family":"Xie","sequence":"additional","affiliation":[{"name":"School of Public Health and Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China"},{"name":"Department of Identification of Traditional Chinese Medicine and Department of Pharmacology, The Second Military Medical University, Shanghai 200433, China"},{"name":"Third Xiangya Hospital and the Institute of Vascular Disease and Translational Medicine, Central South University, Changsha, Hunan 410013, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2018,8,30]]},"reference":[{"key":"2021113010545946700_B1","doi-asserted-by":"publisher","first-page":"1747","DOI":"10.1016\/S0140-6736(06)68770-9","article-title":"Global and regional burden of disease and risk factors, 2001: systematic analysis of population health data","volume":"367","author":"Lopez","year":"2006","journal-title":"Lancet"},{"key":"2021113010545946700_B2","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1007\/s12975-011-0127-8","article-title":"A step-up approach for cell therapy in stroke: translational hurdles of bone marrow-derived stem cells","volume":"3","author":"Glover","year":"2012","journal-title":"Transl. Stroke. Res"},{"key":"2021113010545946700_B3","doi-asserted-by":"publisher","first-page":"3754","DOI":"10.1161\/STR.0000000000000046","article-title":"Guidelines for the primary prevention of stroke: a guideline for healthcare professionals from the American Heart Association\/American Stroke Association","volume":"45","author":"Meschia","year":"2014","journal-title":"Stroke"},{"key":"2021113010545946700_B4","doi-asserted-by":"publisher","first-page":"4207","DOI":"10.1172\/JCI36858","article-title":"Manganese superoxide dismutase expression in endothelial progenitor cell accelerates wound healing in diabetic mice","volume":"120","author":"Marrotte","year":"2010","journal-title":"J. Clin. Invest."},{"key":"2021113010545946700_B5","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1146\/annurev.med.56.090203.104149","article-title":"Endothelial progenitor cells","volume":"56","author":"Khakoo","year":"2005","journal-title":"Annu. Rev. Med"},{"key":"2021113010545946700_B6","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1002\/ana.21919","article-title":"Endothelial progenitor cell transplantation improves long-term stroke outcome in mice","volume":"67","author":"Fan","year":"2010","journal-title":"Ann. Neurol."},{"key":"2021113010545946700_B7","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1159\/000452581","article-title":"Prolonged fasting improves endothelial progenitor cell-mediated ischemic angiogenesis in mice","volume":"40","author":"Xin","year":"2016","journal-title":"Cell. Physiol. Biochem."},{"key":"2021113010545946700_B8","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1161\/HYPERTENSIONAHA.110.160622","article-title":"GTP cyclohydrolase I\/BH4 pathway protects EPCs via suppressing oxidative stress and thrombospondin-1 in salt-sensitive hypertension","volume":"56","author":"Xie","year":"2010","journal-title":"Hypertension"},{"key":"2021113010545946700_B9","doi-asserted-by":"publisher","first-page":"1817","DOI":"10.1161\/STROKEAHA.116.012995","article-title":"Clinical outcomes of transplanted modified bone marrow-derived mesenchymal stem cells in stroke: a phase 1\/2a study","volume":"47","author":"Steinberg","year":"2016","journal-title":"Stroke"},{"key":"2021113010545946700_B10","first-page":"114","article-title":"Stem cell transplantation in the normal nonmyeloablated host: relationship between cell dose, schedule, and engraftment","volume":"25","author":"Rao","year":"1997","journal-title":"Exp. Hematol."},{"key":"2021113010545946700_B11","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1038\/jcbfm.2010.165","article-title":"Reduced ischemic brain injury by partial rejuvenation of bone marrow cells in aged rats","volume":"31","author":"Taguchi","year":"2011","journal-title":"J. Cereb. Blood Flow. Metab"},{"key":"2021113010545946700_B12","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1161\/01.STR.7.1.46","article-title":"Pathogenic similarity of strokes in stroke-prone spontaneously hypertensive rats and humans","volume":"7","author":"Yamori","year":"1976","journal-title":"Stroke"},{"key":"2021113010545946700_B13","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1038\/cr.2013.69","article-title":"ALDH2 protects against stroke by clearing 4-HNE","volume":"23","author":"Guo","year":"2013","journal-title":"Cell Res."},{"key":"2021113010545946700_B14","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.1161\/HYPERTENSIONAHA.111.183368","article-title":"Endothelin 1 activation of endothelin A receptor\/NADPH oxidase pathway and diminished antioxidants critically contribute to endothelial progenitor cell reduction and dysfunction in salt-sensitive hypertention","volume":"59","author":"Chen","year":"2012","journal-title":"Hypertension"},{"key":"2021113010545946700_B15","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1161\/hh0302.104460","article-title":"Bone marrow-derived endothelial progenitor cells participate in cerebral neovascularization after focal cerebral ischemia in the adult mouse","volume":"90","author":"Zhang","year":"2002","journal-title":"Circ. Res."},{"key":"2021113010545946700_B16","doi-asserted-by":"publisher","first-page":"1254","DOI":"10.1161\/STROKEAHA.107.500801","article-title":"Insulin growth factor-1 gene transfer enhances neurovascular remodeling and improves long-term stroke outcome in mice","volume":"39","author":"Zhu","year":"2008","journal-title":"Stroke"},{"key":"2021113010545946700_B17","doi-asserted-by":"publisher","first-page":"1714","DOI":"10.1161\/STROKEAHA.114.007308","article-title":"Dietary intake of sugar substitutes aggravates cerebral ischemic injury and impairs endothelial progenitor cells in mice","volume":"46","author":"Dong","year":"2015","journal-title":"Stroke"},{"key":"2021113010545946700_B18","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1038\/sj.jcbfm.9600034","article-title":"Atorvastatin induction of VEGF and BDNF promotes brain plasticity after stroke in mice","volume":"25","author":"Chen","year":"2005","journal-title":"J. Cereb. Blood Flow. Metab"},{"key":"2021113010545946700_B19","doi-asserted-by":"publisher","first-page":"1972","DOI":"10.1161\/01.STR.29.9.1972","article-title":"Neuronal damage and plasticity identified by microtubule-associated protein 2, growth-associated protein 43, and cyclin D1 immunoreactivity after focal cerebral ischemia in rats","volume":"29","author":"Li","year":"1998","journal-title":"Stroke"},{"key":"2021113010545946700_B20","doi-asserted-by":"publisher","first-page":"e73244","DOI":"10.1371\/journal.pone.0073244","article-title":"Factors secreted by endothelial progenitor cells enhance neurorepair responses after cerebral ischemia in mice","volume":"8","author":"Rosell","year":"2013","journal-title":"PLoS ONE"},{"key":"2021113010545946700_B21","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/j.ebiom.2017.09.002","article-title":"Chronic exposure to subtherapeutic antibiotics aggravates ischemic stroke outcome in mice","volume":"24","author":"Dong","year":"2017","journal-title":"EBiomedicine"},{"key":"2021113010545946700_B22","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1016\/S0002-9440(10)64964-4","article-title":"Hypoxia-induced vascular endothelial growth factor expression precedes neovascularization after cerebral ischemia","volume":"156","author":"Marti","year":"2000","journal-title":"Am. J. Pathol."}],"container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/132\/16\/1797\/450231\/cs-2018-0360.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/132\/16\/1797\/450231\/cs-2018-0360.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T11:46:38Z","timestamp":1638272798000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/132\/16\/1797\/71701\/A-preventive-injection-of-endothelial-progenitor"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,30]]},"references-count":22,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2018,8,31]]}},"URL":"https:\/\/doi.org\/10.1042\/cs20180360","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,30]]}}}