{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:56:25Z","timestamp":1788886585231,"version":"build-2803163510"},"reference-count":39,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,2,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>MiRNAs regulate the cardiomyocyte (CM) cell cycle at the post-transcriptional level, affect cell proliferation, and intervene in harmed CM repair post-injury. The present study was undertaken to characterize the role of let-7i-5p in the processes of CM cell cycle and proliferation and to reveal the mechanisms thereof. In the present study, we used real-time qPCR (RT-qPCR) to determine the up-regulated let-7i-5p in CMs during the postnatal switch from proliferation to terminal differentiation and further validated the role of let-7i-5p by loss- and gain-of-function of let-7i-5p in CMs in vitro and in vivo. We found that the overexpression of let-7i-5p inhibited CM proliferation, whereas the suppression of let-7i-5p significantly facilitated CM proliferation. E2F2 and CCND2 were identified as the targets of let-7i-5p, mediating its effect in regulating the cell cycle of CMs. Supperession of let-7i-5p promoted the recovery of heart function post-myocardial infarction by enhancing E2F2 and CCND2. Collectively, our results revealed that let-7i-5p is involved in the regulation of the CM cell cycle and further impacts proliferation, which may offer a new potential therapeutic strategy for cardiac repair after ischemic injury.<\/jats:p>","DOI":"10.1042\/cs20181002","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T18:55:37Z","timestamp":1548356137000},"page":"425-441","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Suppression of miRNA let-7i-5p promotes cardiomyocyte proliferation and repairs heart function post injury by targetting CCND2 and E2F2"],"prefix":"10.1042","volume":"133","author":[{"given":"Yinlan","family":"Hu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqing","family":"Jin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"},{"name":"Department of Intensive Care Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6822-6063","authenticated-orcid":false,"given":"Yanmei","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lintao","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guojun","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqiang","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayuan","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wangjun","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulin","family":"Liao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuegang","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Bin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research, Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2019,2,5]]},"reference":[{"key":"2021113011093028900_B1","doi-asserted-by":"publisher","first-page":"1035","DOI":"10.1126\/science.aam5894","article-title":"Cardiac regeneration strategies: staying young at heart","volume":"356","author":"Tzahor","year":"2017","journal-title":"Science"},{"key":"2021113011093028900_B2","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1152\/physrev.00032.2006","article-title":"Cardiac myocyte cell cycle control in development, disease, and regeneration","volume":"87","author":"Ahuja","year":"2007","journal-title":"Physiol. 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