{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T02:22:37Z","timestamp":1789698157781,"version":"4.0.1"},"reference-count":45,"publisher":"Portland Press Ltd.","issue":"11","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,6,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Chronic kidney disease (CKD) substantially reduces quality of life and leads to premature death for thousands of people each year. Dialysis and kidney organ transplants remain prevalent therapeutic avenues but carry significant medical, economic and social burden. Podocytes are responsible for blood filtration selectivity in the kidney, where they extend a network of foot processes (FPs) from their cell bodies which surround endothelial cells and interdigitate with those on neighbouring podocytes to form narrow slit diaphragms (SDs). During aging, some podocytes are lost naturally but accelerated podocyte loss is a hallmark of CKD. Insights into the origin of degenerative podocyte loss will help answer important questions about kidney function and lead to substantial health benefits. Here, approaches that uncover insights into podocyte mechanobiology are reviewed, both those that interrogate the biophysical properties of podocytes and how the external physical environment affects podocyte behaviour, and also those that interrogate the biophysical effects that podocytes exert on their surroundings.<\/jats:p>","DOI":"10.1042\/cs20190764","type":"journal-article","created":{"date-parts":[[2020,6,6]],"date-time":"2020-06-06T04:16:14Z","timestamp":1591416974000},"page":"1245-1253","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["The mechanobiology of kidney podocytes in health and disease"],"prefix":"10.1042","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8738-1245","authenticated-orcid":false,"given":"Paul\u00a0A.","family":"Reynolds","sequence":"first","affiliation":[{"name":"School of Medicine, University of St Andrews, St Andrews, KY16 9TF, U.K."},{"name":"Biomedical Sciences Research Complex (BSRC), University of St Andrews, St Andrews, U.K."},{"name":"Centre of Biophotonics, University of St Andrews, St Andrews, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","published-online":{"date-parts":[[2020,6,5]]},"reference":[{"key":"2021113011125313600_B1","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.devcel.2011.06.015","article-title":"A hitchhiker's guide to mechanobiology","volume":"21","author":"Eyckmans","year":"2011","journal-title":"Dev. Cell"},{"key":"2021113011125313600_B2","doi-asserted-by":"publisher","first-page":"162","DOI":"10.3389\/fbioe.2019.00162","article-title":"Innovative Tools for Mechanobiology: Unraveling Outside-In and Inside-Out Mechanotransduction","volume":"7","author":"Mohammed","year":"2019","journal-title":"Front. Bioeng. Biotechnol."},{"key":"2021113011125313600_B3","doi-asserted-by":"publisher","first-page":"eaap8030","DOI":"10.1126\/sciadv.aap8030","article-title":"Podocyte injury elicits loss and recovery of cellular forces","volume":"4","author":"Haley","year":"2018","journal-title":"Sci. Adv."},{"key":"2021113011125313600_B4","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24291","article-title":"The Inability of Podocytes to Proliferate: Cause, Consequences, and Origin","author":"Kriz","year":"2019","journal-title":"Anat. Rec. (Hoboken)"},{"key":"2021113011125313600_B5","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1152\/physrev.00055.2006","article-title":"Properties of the glomerular barrier and mechanisms of proteinuria","volume":"88","author":"Haraldsson","year":"2008","journal-title":"Physiol. Rev."},{"key":"2021113011125313600_B6","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1159\/000368493","article-title":"Rheological influence upon the glomerular podocyte and resultant mechanotransduction","volume":"40","author":"Pichler Sekulic","year":"2015","journal-title":"Kidney Blood Press. Res."},{"key":"2021113011125313600_B7","doi-asserted-by":"publisher","first-page":"F856","DOI":"10.1152\/ajprenal.00196.2005","article-title":"Podocytes are sensitive to fluid shear stress in vitro","volume":"291","author":"Friedrich","year":"2006","journal-title":"Am. J. Physiol. Renal. Physiol."},{"key":"2021113011125313600_B8","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1007\/s12079-010-0088-9","article-title":"Prostaglandin E(2) is crucial in the response of podocytes to fluid flow shear stress","volume":"4","author":"Srivastava","year":"2010","journal-title":"J. Cell Commun. Signal."},{"key":"2021113011125313600_B9","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1016\/S0140-6736(18)33000-9","article-title":"Glomerular podocytes in kidney health and disease","volume":"393","author":"Assady","year":"2019","journal-title":"Lancet"},{"key":"2021113011125313600_B10","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1159\/000481633","article-title":"A Review of Podocyte Biology","volume":"47","author":"Garg","year":"2018","journal-title":"Am. J. Nephrol."},{"key":"2021113011125313600_B11","doi-asserted-by":"publisher","first-page":"332","DOI":"10.2215\/CJN.05000516","article-title":"Minimal Change Disease","volume":"12","author":"Vivarelli","year":"2017","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"2021113011125313600_B12","doi-asserted-by":"publisher","first-page":"626","DOI":"10.2215\/CJN.05810513","article-title":"Glomerular diseases: FSGS","volume":"9","author":"Bose","year":"2014","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"2021113011125313600_B13","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.2215\/CJN.04320416","article-title":"Viral-Associated GN: Hepatitis C and HIV","volume":"12","author":"Kupin","year":"2017","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"2021113011125313600_B14","doi-asserted-by":"publisher","first-page":"609","DOI":"10.2215\/CJN.04160413","article-title":"Glomerular diseases: membranous nephropathy\u2013a modern view","volume":"9","author":"Ponticelli","year":"2014","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"2021113011125313600_B15","doi-asserted-by":"publisher","first-page":"2032","DOI":"10.2215\/CJN.11491116","article-title":"Diabetic Kidney Disease: Challenges, Progress, and Possibilities","volume":"12","author":"Alicic","year":"2017","journal-title":"Clin. J. Am. Soc. Nephrol."},{"key":"2021113011125313600_B16","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.bbrc.2011.02.022","article-title":"Glomerular podocytes: a study of mechanical properties and mechano-chemical signaling","volume":"406","author":"Eekhoff","year":"2011","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021113011125313600_B17","doi-asserted-by":"publisher","first-page":"1517","DOI":"10.1073\/pnas.1717870115","article-title":"Disease-causing mutation in alpha-actinin-4 promotes podocyte detachment through maladaptation to periodic stretch","volume":"115","author":"Feng","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2021113011125313600_B18","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1097\/00041552-200105000-00006","article-title":"Update in podocyte biology","volume":"10","author":"Endlich","year":"2001","journal-title":"Curr. Opin. Nephrol. Hypertens."},{"key":"2021113011125313600_B19","doi-asserted-by":"publisher","first-page":"F693","DOI":"10.1152\/ajprenal.00331.2003","article-title":"p38 MAP kinase mediates mechanically induced COX-2 and PG EP4 receptor expression in podocytes: implications for the actin cytoskeleton","volume":"286","author":"Martineau","year":"2004","journal-title":"Am. J. Physiol. Renal. Physiol."},{"key":"2021113011125313600_B20","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1111\/j.1523-1755.2004.00362.x","article-title":"Activation of a local tissue angiotensin system in podocytes by mechanical strain","volume":"65","author":"Durvasula","year":"2004","journal-title":"Kidney Int."},{"key":"2021113011125313600_B21","doi-asserted-by":"publisher","first-page":"14450","DOI":"10.1096\/fj.201900978RR","article-title":"Fibronectin is up-regulated in podocytes by mechanical stress","volume":"33","author":"Kliewe","year":"2019","journal-title":"FASEB J."},{"key":"2021113011125313600_B22","doi-asserted-by":"publisher","first-page":"9916","DOI":"10.1038\/s41598-017-10116-4","article-title":"Studying the role of fascin-1 in mechanically stressed podocytes","volume":"7","author":"Kliewe","year":"2017","journal-title":"Sci. Rep."},{"key":"2021113011125313600_B23","doi-asserted-by":"publisher","first-page":"84","DOI":"10.14715\/cmb\/2019.65.1.15","article-title":"Upregulation of Tumor Susceptibility Gene 101 (TSG101) by mechanical stress in podocytes","volume":"65","author":"Sunohara","year":"2019","journal-title":"Cell. Mol. Biol. (Noisy-le-grand)"},{"key":"2021113011125313600_B24","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.prostaglandins.2012.11.001","article-title":"Fluid flow shear stress upregulates prostanoid receptor EP2 but not EP4 in murine podocytes","volume":"104-105","author":"Srivastava","year":"2013","journal-title":"Prostaglandins Other Lipid Mediat."},{"key":"2021113011125313600_B25","doi-asserted-by":"publisher","first-page":"F22","DOI":"10.1152\/ajprenal.00325.2017","article-title":"Mechanotransduction signaling in podocytes from fluid flow shear stress","volume":"314","author":"Srivastava","year":"2018","journal-title":"Am. J. Physiol. Renal. Physiol."},{"key":"2021113011125313600_B26","doi-asserted-by":"publisher","first-page":"e0167924","DOI":"10.1371\/journal.pone.0167924","article-title":"Biochemical and Cellular Determinants of Renal Glomerular Elasticity","volume":"11","author":"Embry","year":"2016","journal-title":"PLoS ONE"},{"key":"2021113011125313600_B27","doi-asserted-by":"publisher","first-page":"32623","DOI":"10.1021\/acsami.9b09337","article-title":"Influence of Hydrolyzed Polyacrylamide Hydrogel Stiffness on Podocyte Morphology, Phenotype, and Mechanical Properties","volume":"11","author":"Abdallah","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2021113011125313600_B28","doi-asserted-by":"publisher","first-page":"43934","DOI":"10.1038\/srep43934","article-title":"A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction","volume":"7","author":"Hu","year":"2017","journal-title":"Sci. Rep."},{"key":"2021113011125313600_B29","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aaf8165","article-title":"YAP-mediated mechanotransduction determines the podocyte's response to damage","volume":"10","author":"Rinschen","year":"2017","journal-title":"Sci. Signal"},{"key":"2021113011125313600_B30","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1681\/ASN.2017050475","article-title":"Similar Biophysical Abnormalities in Glomeruli and Podocytes from Two Distinct Models","volume":"29","author":"Embry","year":"2018","journal-title":"J. Am. Soc. Nephrol."},{"key":"2021113011125313600_B31","doi-asserted-by":"publisher","first-page":"3112","DOI":"10.1039\/C8LC00495A","article-title":"Curvature facilitates podocyte culture in a biomimetic platform","volume":"18","author":"Korolj","year":"2018","journal-title":"Lab Chip"},{"key":"2021113011125313600_B32","doi-asserted-by":"publisher","first-page":"4957","DOI":"10.2147\/IJN.S110201","article-title":"A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture","volume":"11","author":"Zennaro","year":"2016","journal-title":"Int. J. Nanomed."},{"key":"2021113011125313600_B33","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-019-4203-8","article-title":"Nephrotic syndrome in a dish: recent developments in modeling in vitro","author":"Veissi","year":"2019","journal-title":"Pediatr. Nephrol."},{"key":"2021113011125313600_B34","doi-asserted-by":"publisher","first-page":"258","DOI":"10.1681\/ASN.2014030278","article-title":"A potential role for mechanical forces in the detachment of podocytes and the progression of CKD","volume":"26","author":"Kriz","year":"2015","journal-title":"J. Am. Soc. Nephrol."},{"key":"2021113011125313600_B35","doi-asserted-by":"publisher","first-page":"379","DOI":"10.3389\/fendo.2018.00379","article-title":"Novel Microscopic Techniques for Podocyte Research","volume":"9","author":"Siegerist","year":"2018","journal-title":"Front Endocrinol. (Lausanne)"},{"key":"2021113011125313600_B36","doi-asserted-by":"crossref","first-page":"3703","DOI":"10.1021\/acsbiomaterials.8b01206","article-title":"Where No Hand Has Gone Before: Probing Mechanobiology at the Cellular Level","volume":"5","author":"Matellan","year":"2019","journal-title":"ACS Biomaterials Sci. Eng."},{"key":"2021113011125313600_B37","doi-asserted-by":"publisher","first-page":"1721","DOI":"10.1039\/C8SM01585F","article-title":"Combined atomic force microscopy (AFM) and traction force microscopy (TFM) reveals a correlation between viscoelastic material properties and contractile prestress of living cells","volume":"15","author":"Schierbaum","year":"2019","journal-title":"Soft Matter"},{"key":"2021113011125313600_B38","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/j.cmet.2010.08.015","article-title":"Insulin signaling to the glomerular podocyte is critical for normal kidney function","volume":"12","author":"Welsh","year":"2010","journal-title":"Cell Metab."},{"key":"2021113011125313600_B39","doi-asserted-by":"publisher","first-page":"F701","DOI":"10.1152\/ajprenal.00246.2006","article-title":"HIV infection changes glomerular podocyte cytoskeletal composition and results in distinct cellular mechanical properties","volume":"292","author":"Tandon","year":"2007","journal-title":"Am. J. Physiol. Renal. Physiol."},{"key":"2021113011125313600_B40","doi-asserted-by":"publisher","first-page":"C397","DOI":"10.1152\/ajpcell.00438.2010","article-title":"Biophysical properties of normal and diseased renal glomeruli","volume":"300","author":"Wyss","year":"2011","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"2021113011125313600_B41","doi-asserted-by":"publisher","first-page":"7857","DOI":"10.1021\/acs.nanolett.6b04124","article-title":"Single Particle Nanoplasmonic Sensing in Individual Nanofluidic Channels","volume":"16","author":"Fritzsche","year":"2016","journal-title":"Nano Lett."},{"key":"2021113011125313600_B42","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1038\/ncb3277","article-title":"Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft matrices","volume":"18","author":"Wolfenson","year":"2016","journal-title":"Nat. Cell Biol."},{"key":"2021113011125313600_B43","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1038\/ncb3134","article-title":"Force transmission during adhesion-independent migration","volume":"17","author":"Bergert","year":"2015","journal-title":"Nat. Cell Biol."},{"key":"2021113011125313600_B44","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1038\/s41598-018-36896-x","article-title":"Traction force microscopy with optimized regularization and automated Bayesian parameter selection for comparing cells","volume":"9","author":"Huang","year":"2019","journal-title":"Sci. Rep."},{"key":"2021113011125313600_B45","doi-asserted-by":"publisher","first-page":"864","DOI":"10.1038\/ncb3561","article-title":"Long-term imaging of cellular forces with high precision by elastic resonator interference stress microscopy","volume":"19","author":"Kronenberg","year":"2017","journal-title":"Nat. Cell Biol."}],"container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/134\/11\/1245\/883317\/cs-2019-0764c.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/134\/11\/1245\/883317\/cs-2019-0764c.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T07:11:47Z","timestamp":1638256307000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/134\/11\/1245\/225170\/The-mechanobiology-of-kidney-podocytes-in-health"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6]]},"references-count":45,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,6,12]]}},"URL":"https:\/\/doi.org\/10.1042\/cs20190764","relation":{},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,6]]},"published":{"date-parts":[[2020,6]]}}}