{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T23:30:49Z","timestamp":1775086249796,"version":"3.50.1"},"reference-count":46,"publisher":"Portland Press Ltd.","issue":"20","license":[{"start":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T00:00:00Z","timestamp":1633392000000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,10,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>One of the health benefits of endurance exercise training (ET) is the stimulation of hematopoiesis. However, the mechanisms underlying ET-induced hematopoietic adaptations are understudied. N\u2013Acetyl\u2013Seryl\u2013Aspartyl\u2013Lysyl\u2013Proline (Ac-SDKP) inhibits proliferation of early hematopoietic progenitor cells. The angiotensin I-converting enzyme (ACE) NH2-terminal promotes hematopoiesis by inhibiting the anti-hematopoietic effect of Ac-SDKP. Here we demonstrate for the first time the role of ACE NH2-terminal in ET-induced hematopoietic adaptations. Wistar rats were subjected to 10 weeks of moderate-(T1) and high-(T2) volume swimming-training. Although both protocols induced classical ET-associated adaptations, only T2 increased plasma ACE NH2-domain activity (by 40%, P=0.0003) and reduced Ac-SDKP levels (by 50%, P&amp;lt;0.0001). T2 increased the number of hematopoietic stem cells (HSCs; \u223c200%, P=0.0008), early erythroid progenitor colonies (\u223c300%, P&amp;lt;0.0001) and reticulocytes (\u223c500%, P=0.0007), and reduced erythrocyte lifespan (\u223c50%, P=0.022). Following, Wistar rats were subjected to T2 or T2 combined with ACE NH2-terminal inhibition (captopril (Cap) treatment: 10 mg.kg\u22121.day\u22121). T2 combined with ACE NH2-terminal inhibition prevented Ac-SDKP decrease and attenuated ET-induced hematopoietic adaptations. Altogether, our findings show that ET-induced hematopoiesis was at least partially associated with increased ACE NH2-terminal activity and reduction in the hematopoietic inhibitor Ac-SDKP.<\/jats:p>","DOI":"10.1042\/cs20210739","type":"journal-article","created":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T21:11:57Z","timestamp":1633468317000},"page":"2377-2391","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["High-volume endurance exercise training stimulates hematopoiesis by increasing ACE NH2-terminal activity"],"prefix":"10.1042","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1963-982X","authenticated-orcid":false,"given":"Fl\u00e1vio\u00a0de\u00a0Castro","family":"Magalh\u00e3es","sequence":"first","affiliation":[{"name":"Laboratory of Biochemistry of the Motor Activity, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil"},{"name":"Laboratory of Exercise Biology, Integrated Center of Health Research, Programa Multic\u00eantrico de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Fisiol\u00f3gicas-Federal University of the Jequitinhonha and Mucuri Valleys, 5000 MGT 367 Road-km 583-Alto da Jacuba, 39100-000, Diamantina, Minas Gerais, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiago","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry of the Motor Activity, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vin\u00edcius","family":"Bassaneze","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), Medical School, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katt\u00a0Coelho","family":"Mattos","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry of the Motor Activity, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isolmar","family":"Schettert","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), Medical School, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio\u00a0Luiz\u00a0Navarro","family":"Marques","sequence":"additional","affiliation":[{"name":"Radiopharmacy Laboratory, Nuclear Medicine Center, Medical School, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5464-1792","authenticated-orcid":false,"given":"Jos\u00e9\u00a0Eduardo","family":"Krieger","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics and Molecular Cardiology, Heart Institute (InCor), Medical School, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Nava","sequence":"additional","affiliation":[{"name":"Department of Health, Exercise, and Sports Sciences, University of New Mexico, Albuquerque, NM 87131, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val\u00e9rio\u00a0Garrone","family":"Barauna","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Physiology, Health Sciences Center, Federal University of Esp\u00edrito Santo, Vit\u00f3ria, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliveira","family":"Edilamar\u00a0Menezes","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry of the Motor Activity, School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2021,10,19]]},"reference":[{"key":"2021121410073205900_B1","doi-asserted-by":"publisher","first-page":"4677","DOI":"10.1113\/JP270250","article-title":"Haematological rather than skeletal muscle adaptations contribute to the increase in peak oxygen uptake induced by moderate endurance training","volume":"593","author":"Montero","year":"2015","journal-title":"J. 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