{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T06:22:13Z","timestamp":1772691733130,"version":"3.50.1"},"reference-count":47,"publisher":"Portland Press Ltd.","issue":"11","license":[{"start":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T00:00:00Z","timestamp":1653004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"}],"content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,6,17]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Changes to some Golgi subfamily member proteins are reported to be involved in tumor metastasis. However, the functional role and potential mechanism of the Golgi A8 family member B (GOLGA8B) in lung squamous cell carcinoma (LUSC) remains unknown. In the present study, GOLGA8B expression was detected using qRT-PCR, Western blot, and immunohistochemistry (IHC). In vivo animal experiments and in vitro functional assays were performed to explore the function of GOLGA8B in LUSC. Luciferase assays were performed to investigate the underlying targets of GOLGA8B in LUSC. GOLGA8B was shown to be highly expressed in LUSC metastasis tissue, and significantly associated with the distant metastasis-free survival of LUSC patients. Loss-of-function assays indicated that silencing GOLGA8B suppressed LUSC cell tumorigenesis in vivo and weakened in vitro invasion and migration. GOLGA8B silencing-induced inhibition of invasion and migration was associated with the inactivation of STAT3 signaling. Importantly, these results showed that the number of circulating tumor cells (CTCs) was markedly higher in the GOLGA8B silencing group than in the control vector group. GOLGA8B expression was positively associated with p-STAT3 expression in LUSC tissue. Study findings revealed a novel mechanism by which GOLGA8B promotes tumor metastasis in LUSC cells and suggests that this protein could be a promising target for antitumor metastasis therapy in LUSC patients.<\/jats:p>","DOI":"10.1042\/cs20220128","type":"journal-article","created":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T08:35:06Z","timestamp":1653035706000},"page":"895-909","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Silencing GOLGA8B inhibits cell invasion and metastasis by suppressing STAT3 signaling pathway in lung squamous cell carcinoma"],"prefix":"10.1042","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7965-7608","authenticated-orcid":false,"given":"Zhanzhan","family":"Li","sequence":"first","affiliation":[{"name":"1Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanyan","family":"Li","sequence":"additional","affiliation":[{"name":"2Department of Nursing, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"1Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liangfang","family":"Shen","sequence":"additional","affiliation":[{"name":"1Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1600-8288","authenticated-orcid":false,"given":"Aibin","family":"Liu","sequence":"additional","affiliation":[{"name":"3Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China"},{"name":"4National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha 410008, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2022,6,6]]},"reference":[{"key":"2022060611131228800_B1","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1002\/jso.24532","article-title":"Lung cancer-a global perspective","volume":"115","author":"McIntyre","year":"2017","journal-title":"J. Surg. Oncol."},{"key":"2022060611131228800_B2","doi-asserted-by":"publisher","DOI":"10.5334\/aogh.2419","article-title":"Global epidemiology of lung cancer","volume":"85","author":"Barta","year":"2019","journal-title":"Ann. Glob. Health"},{"key":"2022060611131228800_B3","doi-asserted-by":"publisher","first-page":"29","DOI":"10.33594\/000000340","article-title":"Prognosis of different types of non-small cell lung cancer progression: current state and perspectives","volume":"55","author":"Schegoleva","year":"2021","journal-title":"Cell. Physiol. Biochem."},{"key":"2022060611131228800_B4","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/978-3-319-40389-2_3","article-title":"Lung cancer staging and prognosis","volume":"170","author":"Woodard","year":"2016","journal-title":"Cancer Treat. Res."},{"key":"2022060611131228800_B5","doi-asserted-by":"publisher","first-page":"R229","DOI":"10.1016\/j.cub.2019.01.003","article-title":"Protein glycosylation","volume":"29","author":"Eichler","year":"2019","journal-title":"Curr. Biol."},{"key":"2022060611131228800_B6","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1146\/annurev-pathol-012414-040438","article-title":"Protein glycosylation in cancer","volume":"10","author":"Stowell","year":"2015","journal-title":"Annu. Rev. Pathol."},{"key":"2022060611131228800_B7","doi-asserted-by":"publisher","first-page":"157","DOI":"10.3389\/fcell.2019.00157","article-title":"The N-glycosylation processing potential of the mammalian golgi apparatus","volume":"7","author":"Fisher","year":"2019","journal-title":"Front. Cell Dev. Biol."},{"key":"2022060611131228800_B8","doi-asserted-by":"publisher","first-page":"247","DOI":"10.3390\/genes10030247","article-title":"Golgi oncoprotein GOLPH3 gene expression is regulated by functional E2F and CREB\/ATF promoter elements","volume":"10","author":"Penalver-Gonzalez","year":"2019","journal-title":"Genes (Basel)"},{"key":"2022060611131228800_B9","doi-asserted-by":"publisher","first-page":"2052","DOI":"10.1038\/mt.2012.125","article-title":"GOLGA2\/GM130, cis-Golgi matrix protein, is a novel target of anticancer gene therapy","volume":"20","author":"Chang","year":"2012","journal-title":"Mol. Ther."},{"key":"2022060611131228800_B10","doi-asserted-by":"publisher","first-page":"1940","DOI":"10.1172\/JCI123089","article-title":"Profound MEK inhibitor response in a cutaneous melanoma harboring a GOLGA4-RAF1 fusion","volume":"129","author":"McEvoy","year":"2019","journal-title":"J. Clin. Invest."},{"key":"2022060611131228800_B11","doi-asserted-by":"publisher","DOI":"10.1172\/JCI137186","article-title":"A protumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma","volume":"131","author":"Tan","year":"2021","journal-title":"J. Clin. Invest."},{"key":"2022060611131228800_B12","doi-asserted-by":"publisher","first-page":"2052","DOI":"10.1038\/mt.2012.125","article-title":"GOLGA2\/GM130, cis-Golgi matrix protein, is a novel target of anticancer gene therapy","volume":"20","author":"Chang","year":"2012","journal-title":"Mol. Ther."},{"key":"2022060611131228800_B13","doi-asserted-by":"publisher","first-page":"e211501","DOI":"10.1371\/journal.pone.0211501","article-title":"Golgin-160 and GMAP210 play an important role in U251 cells migration and invasion initiated by GDNF","volume":"14","author":"Tang","year":"2019","journal-title":"PloS ONE"},{"key":"2022060611131228800_B14","doi-asserted-by":"publisher","first-page":"8006","DOI":"10.1111\/jcmm.15432","article-title":"Identification of castration-resistant prostate cancer-related hub genes using weighted gene co-expression network analysis","volume":"24","author":"Cheng","year":"2020","journal-title":"J. Cell. Mol. Med."},{"key":"2022060611131228800_B15","first-page":"12576","article-title":"Construction and comprehensive analysis of dysregulated long non-coding RNA-associated competing endogenous RNA network in clear cell renal cell carcinoma","volume":"120","author":"Wang","year":"2018","journal-title":"J. Cell. Biochem."},{"key":"2022060611131228800_B16","doi-asserted-by":"publisher","first-page":"544","DOI":"10.3390\/cells8060544","article-title":"Glycosylation in the tumor microenvironment: implications for tumor angiogenesis and metastasis","volume":"8","author":"Chandler","year":"2019","journal-title":"Cells-Basel"},{"key":"2022060611131228800_B17","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1007\/s10555-020-09921-7","article-title":"A systems biology approach to discovering pathway signaling dysregulation in metastasis","volume":"39","author":"Clarke","year":"2020","journal-title":"Cancer Metastasis Rev."},{"key":"2022060611131228800_B18","doi-asserted-by":"publisher","first-page":"421821","DOI":"10.1155\/2013\/421821","article-title":"Role of STAT3 in cancer metastasis and translational advances","volume":"2013","author":"Kamran","year":"2013","journal-title":"Biomed. Res. Int."},{"key":"2022060611131228800_B19","doi-asserted-by":"publisher","first-page":"217","DOI":"10.3390\/cells9010217","article-title":"Role of JAK\/STAT3 signaling in the regulation of metastasis, the transition of cancer stem cells, and chemoresistance of cancer by epithelial-mesenchymal transition","volume":"9","author":"Jin","year":"2020","journal-title":"Cells-Basel"},{"key":"2022060611131228800_B20","doi-asserted-by":"publisher","first-page":"e28086","DOI":"10.4161\/jkst.28086","article-title":"The involvement of JAK-STAT3 in cell motility, invasion, and metastasis","volume":"3","author":"Teng","year":"2014","journal-title":"JAKSTAT"},{"key":"2022060611131228800_B21","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1186\/s12943-020-01217-2","article-title":"LINC00173.v1 promotes angiogenesis and progression of lung squamous cell carcinoma by sponging miR-511-5p to regulate VEGFA expression","volume":"19","author":"Chen","year":"2020","journal-title":"Mol. Cancer"},{"key":"2022060611131228800_B22","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.canlet.2020.04.007","article-title":"Downregulation of the circadian rhythm regulator HLF promotes multiple-organ distant metastases in non-small cell lung cancer through PPAR\/NF-kappab signaling","volume":"482","author":"Chen","year":"2020","journal-title":"Cancer Lett."},{"key":"2022060611131228800_B23","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1038\/nrclinonc.2018.8","article-title":"Targeting the IL-6\/JAK\/STAT3 signalling axis in cancer","volume":"15","author":"Johnson","year":"2018","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"2022060611131228800_B24","doi-asserted-by":"publisher","first-page":"1293","DOI":"10.1038\/ng.3120","article-title":"Palindromic GOLGA8 core duplicons promote chromosome 15q13.3 microdeletion and evolutionary instability","volume":"46","author":"Antonacci","year":"2014","journal-title":"Nat. Genet."},{"key":"2022060611131228800_B25","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1159\/000443343","article-title":"Clinical and genetic heterogeneity of the 15q13.3 microdeletion syndrome","volume":"6","author":"Hassfurther","year":"2016","journal-title":"Mol. Syndromol."},{"key":"2022060611131228800_B26","doi-asserted-by":"publisher","first-page":"8006","DOI":"10.1111\/jcmm.15432","article-title":"Identification of castration-resistant prostate cancer-related hub genes using weighted gene co-expression network analysis","volume":"24","author":"Cheng","year":"2020","journal-title":"J. Cell. Mol. Med."},{"key":"2022060611131228800_B27","doi-asserted-by":"crossref","first-page":"2576","DOI":"10.1002\/jcb.27557","article-title":"Construction and comprehensive analysis of dysregulated long non-coding RNA-associated competing endogenous RNA network in clear cell renal cell carcinoma","volume":"120","author":"Wang","year":"2018","journal-title":"J. Cell. Biochem."},{"key":"2022060611131228800_B28","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.3390\/cells9041043","article-title":"A STAT3 of addiction: adipose tissue, adipocytokine signalling and STAT3 as mediators of metabolic remodelling in the tumour microenvironment","volume":"9","author":"Kadye","year":"2020","journal-title":"Cells-Basel"},{"key":"2022060611131228800_B29","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1016\/j.semcdb.2008.06.005","article-title":"The function of Stat3 in tumor cells and their microenvironment","volume":"19","author":"Groner","year":"2008","journal-title":"Semin. Cell Dev. Biol."},{"key":"2022060611131228800_B30","doi-asserted-by":"publisher","first-page":"1016","DOI":"10.1084\/jem.20181827","article-title":"Context-dependent EMT programs in cancer metastasis","volume":"216","author":"Aiello","year":"2019","journal-title":"J. Exp. Med."},{"key":"2022060611131228800_B31","doi-asserted-by":"publisher","first-page":"20200087","DOI":"10.1098\/rstb.2020.0087","article-title":"Epithelial-mesenchymal plasticity: emerging parallels between tissue morphogenesis and cancer metastasis","volume":"375","author":"Plygawko","year":"2020","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"2022060611131228800_B32","doi-asserted-by":"publisher","first-page":"3502","DOI":"10.1681\/ASN.2016060603","article-title":"Beyond EMT: epithelial STAT3 as a central regulator of fibrogenesis","volume":"27","author":"Duffield","year":"2016","journal-title":"J. Am. Soc. Nephrol."},{"key":"2022060611131228800_B33","doi-asserted-by":"publisher","first-page":"1658","DOI":"10.1158\/1541-7786.MCR-11-0271","article-title":"IL-6 promotes head and neck tumor metastasis by inducing epithelial-mesenchymal transition via the JAK-STAT3-SNAIL signaling pathway","volume":"9","author":"Yadav","year":"2011","journal-title":"Mol. Cancer Res."},{"key":"2022060611131228800_B34","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.jss.2010.10.030","article-title":"Activation of STAT3 signal pathway correlates with twist and E-cadherin expression in hepatocellular carcinoma and their clinical significance","volume":"174","author":"Zhang","year":"2012","journal-title":"J. Surg. Res."},{"key":"2022060611131228800_B35","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1186\/s12943-019-0959-5","article-title":"Hypoxic BMSC-derived exosomal miRNAs promote metastasis of lung cancer cells via STAT3-induced EMT","volume":"18","author":"Zhang","year":"2019","journal-title":"Mol. Cancer"},{"key":"2022060611131228800_B36","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1016\/j.ccr.2013.04.017","article-title":"Tumor cell dissemination: emerging biological insights from animal models and cancer patients","volume":"23","author":"Kang","year":"2013","journal-title":"Cancer Cell"},{"key":"2022060611131228800_B37","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1016\/j.ccell.2016.03.003","article-title":"An Arntl2-driven secretome enables lung adenocarcinoma metastatic self-sufficiency","volume":"29","author":"Brady","year":"2016","journal-title":"Cancer Cell"},{"key":"2022060611131228800_B38","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/j.ccell.2016.02.013","article-title":"PITPNC1 recruits RAB1B to the Golgi network to drive malignant secretion","volume":"29","author":"Halberg","year":"2016","journal-title":"Cancer Cell"},{"key":"2022060611131228800_B39","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1172\/JCI88736","article-title":"Epithelial-to-mesenchymal transition drives a pro-metastatic Golgi compaction process through scaffolding protein PAQR11","volume":"127","author":"Tan","year":"2017","journal-title":"J. Clin. Invest."},{"key":"2022060611131228800_B40","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.etap.2017.02.001","article-title":"27-Hydroxycholesterol induces invasion and migration of breast cancer cells by increasing MMP9 and generating EMT through activation of STAT-3","volume":"51","author":"Shen","year":"2017","journal-title":"Environ. Toxicol. Pharmacol."},{"key":"2022060611131228800_B41","doi-asserted-by":"publisher","first-page":"e241423","DOI":"10.1371\/journal.pone.0241423","article-title":"Mesenchymal stem cells promote metastasis through activation of an ABL-MMP9 signaling axis in lung cancer cells","volume":"15","author":"Gu","year":"2020","journal-title":"PloS ONE"},{"key":"2022060611131228800_B42","doi-asserted-by":"publisher","first-page":"5785","DOI":"10.1007\/s13277-015-3247-z","article-title":"MiR-129 regulates MMP9 to control metastasis of non-small cell lung cancer","volume":"36","author":"Li","year":"2015","journal-title":"Tumour Biol."},{"key":"2022060611131228800_B43","doi-asserted-by":"publisher","first-page":"11051","DOI":"10.1007\/s13277-014-2409-8","article-title":"SEMA4b inhibits MMP9 to prevent metastasis of non-small cell lung cancer","volume":"35","author":"Jian","year":"2014","journal-title":"Tumour Biol."},{"key":"2022060611131228800_B44","doi-asserted-by":"publisher","first-page":"3883","DOI":"10.3892\/ol.2017.6700","article-title":"Role of liver ICAM-1 in metastasis","volume":"14","author":"Benedicto","year":"2017","journal-title":"Oncol. Lett."},{"key":"2022060611131228800_B45","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.4049\/jimmunol.161.5.2333","article-title":"Protection from lymphoma cell metastasis in ICAM-1 mutant mice: a posthoming event","volume":"161","author":"Aoudjit","year":"1998","journal-title":"J. Immunol."},{"key":"2022060611131228800_B46","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1254\/jphs.92.7","article-title":"The regulation of ICAM-1 and LFA-1 interaction by autacoids and statins: a novel strategy for controlling inflammation and immune responses","volume":"92","author":"Nishibori","year":"2003","journal-title":"J. Pharmacol. Sci."},{"key":"2022060611131228800_B47","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1111\/j.1365-2249.1994.tb06138.x","article-title":"Podocyte expression of MHC class I and II and intercellular adhesion molecule-1 (ICAM-1) in experimental pauci-immune crescentic glomerulonephritis","volume":"98","author":"Coers","year":"1994","journal-title":"Clin. Exp. Immunol."}],"updated-by":[{"DOI":"10.1042\/cs-2022-0128_cor","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T00:00:00Z","timestamp":1723161600000}}],"container-title":["Clinical Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/136\/11\/895\/933438\/cs-2022-0128.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/clinsci\/article-pdf\/136\/11\/895\/933438\/cs-2022-0128.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,5]],"date-time":"2023-02-05T18:56:51Z","timestamp":1675623411000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/clinsci\/article\/136\/11\/895\/231327\/Silencing-GOLGA8B-inhibits-cell-invasion-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":47,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,6,17]]}},"URL":"https:\/\/doi.org\/10.1042\/cs20220128","relation":{"correction":[{"id-type":"doi","id":"10.1042\/CS-2022-0128_COR","asserted-by":"object"}]},"ISSN":["0143-5221","1470-8736"],"issn-type":[{"value":"0143-5221","type":"print"},{"value":"1470-8736","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,6]]},"published":{"date-parts":[[2022,6]]}}}