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Here, we review how mass spectrometry (MS) can be used to study short-term control by AMPK via protein phosphorylation and long-term control due to changes in protein expression. We discuss how MS can quantify AMPK subunit levels in tissues from different species. We propose hydrogen-deuterium exchange (HDX)-MS to investigate molecular mechanisms of AMPK activation and thermoproteomic profiling (TPP) to assess off-target effects of pharmacological AMPK activators\/inhibitors. Lastly, because large MS data sets are generated, we consider different approaches that can be used for their interpretation.<\/jats:p>","DOI":"10.1042\/ebc20240009","type":"journal-article","created":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T08:43:42Z","timestamp":1721983422000},"page":"283-294","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["How mass spectrometry can be exploited to study AMPK"],"prefix":"10.1042","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2655-8921","authenticated-orcid":false,"given":"Mark\u00a0H.","family":"Rider","sequence":"additional","affiliation":[{"name":"Protein Phosphorylation (PHOS) laboratory, Universit\u00e9 catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Didier","family":"Vertommen","sequence":"additional","affiliation":[{"name":"Protein Phosphorylation (PHOS) laboratory, Universit\u00e9 catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Johanns","sequence":"additional","affiliation":[{"name":"Protein Phosphorylation (PHOS) laboratory, Universit\u00e9 catholique de Louvain and de Duve Institute, Avenue Hippocrate 75, B-1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2024,11,18]]},"reference":[{"key":"2024111809362057000_B1","doi-asserted-by":"publisher","first-page":"20170774","DOI":"10.1098\/rsif.2017.0774","article-title":"Keeping the home fires burning: AMP-activated protein kinase","volume":"15","author":"Hardie","year":"2018","journal-title":"J. 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