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Most of the fundamental discoveries have been invariably linked to advances in technology. However, due to their size, delicate structural integrity and their sheer number, our knowledge of synaptic biology has remained somewhat elusive and their role in neurodegenerative diseases still remains largely unknown. Here, we briefly discuss some of the imaging technologies used to study synapses and focus on the utility of the high-resolution imaging technique array tomography (AT). We introduce the AT technique and highlight some of the ways it is utilised with a particular focus on its power for analysing synaptic composition and pathology in human post-mortem tissue. We also discuss some of the benefits and drawbacks of techniques for imaging synapses and highlight some recent advances in the study of form and function by combining physiology and high-resolution synaptic imaging.<\/jats:p>","DOI":"10.1042\/ns20220013","type":"journal-article","created":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T12:40:43Z","timestamp":1662468043000},"update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Array tomography: 15 years of synaptic analysis"],"prefix":"10.1042","volume":"6","author":[{"given":"Anna","family":"Sanchez Avila","sequence":"first","affiliation":[{"name":"1Euan Macdonald Centre for Motor Neuron Disease, Edinburgh, UK"},{"name":"2Division of Cellular and Systems Medicine, University of Dundee, Dundee, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0393-180X","authenticated-orcid":false,"given":"Christopher\u00a0M.","family":"Henstridge","sequence":"additional","affiliation":[{"name":"1Euan Macdonald Centre for Motor Neuron Disease, Edinburgh, UK"},{"name":"2Division of Cellular and Systems Medicine, University of Dundee, Dundee, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","published-online":{"date-parts":[[2022,9,23]]},"reference":[{"key":"2022092309001721400_B1","doi-asserted-by":"publisher","first-page":"1252","DOI":"10.1002\/ar.24392","article-title":"The history of the synapse","volume":"303","author":"Sotelo","year":"2020","journal-title":"Anat. 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