{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T05:21:02Z","timestamp":1773292862913,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2001,4,15]]},"abstract":"<jats:p>Cytochrome b5 reductase (b5R) is an essential enzyme that exists in soluble and membrane-bound isoforms, each with specific functions. In the rat, the two forms are generated from alternative transcripts differing in the first exons. In contrast, the biogenesis of b5R isoforms in the human is not yet well understood. In the present study we have detected three novel alternative exons, designated 1S, S\u2032 and 1B, located between the first alternative exon 1M and the common second exon in the human b5R gene. Accordingly, multiple M-type, S-type and SS\u2032-type and B-type transcripts are generated. All types of human b5R transcript are expressed ubiquitously. An analysis of in vitro translation products demonstrated an alternative use of different AUG initiators resulting in the production of various human b5R protein isoforms. Our results indicate that the organization of the 5\u2032 region of the b5R gene is not conserved between rodents and humans. Insertion of Alu elements into the human b5R gene, in particular just upstream of the S\/S\u2032 region, could be responsible for dynamic events of gene rearrangement during evolution.<\/jats:p>","DOI":"10.1042\/bj3550529","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T22:14:52Z","timestamp":1439244892000},"page":"529-535","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Transcriptional and translational mechanisms of cytochrome b5 reductase isoenzyme generation in humans"],"prefix":"10.1042","volume":"355","author":[{"given":"Alena","family":"LEROUX","sequence":"first","affiliation":[{"name":"Institut Cochin de G\u00e9n\u00e9tique Mol\u00e9culaire, U.129 INSERM Unit\u00e9 de Recherches en Physiologie et Pathologie G\u00e9n\u00e9tiques et Mol\u00e9culaires, Universit\u00e9 Ren\u00e9 Descartes, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France"}]},{"given":"Luisa MOTA","family":"VIEIRA","sequence":"additional","affiliation":[{"name":"Institut Cochin de G\u00e9n\u00e9tique Mol\u00e9culaire, U.129 INSERM Unit\u00e9 de Recherches en Physiologie et Pathologie G\u00e9n\u00e9tiques et Mol\u00e9culaires, Universit\u00e9 Ren\u00e9 Descartes, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France"}]},{"given":"Axel","family":"KAHN","sequence":"additional","affiliation":[{"name":"Institut Cochin de G\u00e9n\u00e9tique Mol\u00e9culaire, U.129 INSERM Unit\u00e9 de Recherches en Physiologie et Pathologie G\u00e9n\u00e9tiques et Mol\u00e9culaires, Universit\u00e9 Ren\u00e9 Descartes, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France"}]}],"member":"288","published-online":{"date-parts":[[2001,4,6]]},"container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/355\/2\/529\/706972\/bj3550529.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/355\/2\/529\/706972\/bj3550529.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T11:08:06Z","timestamp":1637579286000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/355\/2\/529\/38548\/Transcriptional-and-translational-mechanisms-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4,6]]},"references-count":0,"aliases":["10.1042\/0264-6021:3550529"],"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,4,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3550529","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,4,6]]}}}