{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T21:33:07Z","timestamp":1771623187171,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1968,1,1]]},"abstract":"<jats:p>1. The exchangeability with added radioactive acetylcholine of the acetylcholine in isolated presynaptic nerve terminals (synaptosomes) and isolated synaptic vesicles was studied by a Sephadex-column method. 2. A substantial proportion of the synaptosomal acetylcholine is exchangeable with added radioactive acetylcholine. It is liberated by hypo-osmotic shock and ultrasonic treatment, and behaves as though it occupies the cytoplasmic compartment of synaptosomes. 3. Methods of isolating vesicles from hypo-osmotically ruptured synaptosomes in optimum yield are discussed. 4. The acetylcholine of synaptic vesicles isolated on a sucrose density gradient is released by hypo-osmotic conditions, suggesting that it is enclosed by a semi-permeable membrane; however, it is not easily released by ultrasonic treatment. 5. Added radioactive acetylcholine does not exchange with vesicular acetylcholine under a variety of different conditions. These include addition of ATP and Mg2+, and pre-loading of the synaptosome with radioactive acetylcholine before hypo-osmotic rupture. This failure to exchange is discussed in terms of the possible storage mechanism of vesicular acetylcholine.<\/jats:p>","DOI":"10.1042\/bj1060087","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:13:27Z","timestamp":1439234007000},"page":"87-95","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Exchangeability of radioactive acetylcholine with the bound acetylcholine of synaptosomes and synaptic vesicles"],"prefix":"10.1042","volume":"106","author":[{"given":"R. M.","family":"Marchbanks","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Agricultural Research Council Institute of Animal Physiology, Babraham, Cambridge"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/106\/1\/87\/760116\/bj1060087.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/106\/1\/87\/760116\/bj1060087.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T20:49:56Z","timestamp":1638218996000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/106\/1\/87\/3366\/Exchangeability-of-radioactive-acetylcholine-with"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1968,1,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1060087","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1968,1,1]]}}}