{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T21:32:06Z","timestamp":1771623126020,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1968,1,1]]},"abstract":"<jats:p>1. The native hormone-binding proteins, neurophysin-I and -II, have been isolated from acetone-desiccated bovine pituitary posterior lobes. 2. Neurophysin-I and -II are present in approximately equal quantities in the tissue and are localized in the neurosecretory granules. 3. The apparent molecular weight, determined by equilibrium sedimentation of neurophysin-I, was 19000 and that of neurophysin-II was 21000; their sedimentation coefficients, S20,w, were 1\u00b766 and 2\u00b702s respectively. 4. Neurophysin-I and -II are similar in amino acid composition. Neurophysin-II was distinguished from neurophysin-I by the absence of histidine. 5. The proteins form complexes with oxytocin as well as with vasopressin. Complexes of both proteins with [8-arginine]-vasopressin have been crystallized. 6. Bioassay of the pressor and oxytocic activities of the crystals shows that neurophysin-I binds three molecules of either vasopressin or oxytocin whereas neurophysin-II binds only two molecules of each hormone per molecule of protein. Complexes containing two molecules of oxytocin and one molecule of [8-arginine]-vasopressin per molecule of protein are formed by neurophysin-I and -II; both proteins appear to possess three polypeptide-binding sites\/molecule.<\/jats:p>","DOI":"10.1042\/bj1060557","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:13:27Z","timestamp":1439234007000},"page":"557-564","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":103,"title":["The isolation of the native hormone-binding proteins from bovine pituitary posterior lobes. Crystallization of neurophysin-I and -II as complexes with [8-arginine]-vasopressin"],"prefix":"10.1042","volume":"106","author":[{"given":"M D","family":"Hollenberg","sequence":"first","affiliation":[{"name":"Department of Pharmacology, University of Oxford"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D B","family":"Hope","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Oxford"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/106\/2\/557\/760424\/bj1060557.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/106\/2\/557\/760424\/bj1060557.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T20:48:16Z","timestamp":1638218896000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/106\/2\/557\/3327\/The-isolation-of-the-native-hormone-binding"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,1,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1968,1,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1060557","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1968,1,1]]}}}