{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T05:38:23Z","timestamp":1699767503089},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1968,10,1]]},"abstract":"<jats:p>1. The role of disulphide-bond formation in the assembly of G2a myeloma protein 5563 was studied by pulse-labelling ascitic plasma cells of tumour-line 5563 for 2\u20138min. with radioactive amino acids, and analysing the intracellular proteins. Myeloma-protein determinants were first purified by ion-exchange chromatography under conditions that do not dissociate non-covalently linked sub-units of immunoglobulin G. The pulse-labelled material was then analysed by electrophoresis on polyacrylamide gels in sodium dodecyl sulphate\u2013phosphate\u2013urea buffer, which dissociates non-covalently linked sub-units; after gel electrophoresis, radioactive protein bands were located by radioautography, and characterized immunologically after elution. 2. Two heavy-chain intermediates were detected: (i) heavy-chain dimer; (ii) the dimer with one light chain attached. Free light chains had previously been shown to be intermediates in assembly. No evidence for the presence of half-molecules (one light chain attached to one heavy chain) was obtained. The formation of the disulphide bond between the heavy chains thus appears to precede the light-chain\u2013heavy-chain linkage in immunoglobulin G assembly.<\/jats:p>","DOI":"10.1042\/bj1090637","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:36:50Z","timestamp":1439501810000},"page":"637-643","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Interchain disulphide-bond formation in the assembly of immunoglobulin G. Heavy-chain dimer as an intermediate"],"prefix":"10.1042","volume":"109","author":[{"given":"Brigitte A.","family":"Askonas","sequence":"first","affiliation":[{"name":"National Institute for Medical Research, London, N.W. 7"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan R.","family":"Williamson","sequence":"additional","affiliation":[{"name":"National Institute for Medical Research, London, N.W. 7"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/109\/4\/637\/761640\/bj1090637.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/109\/4\/637\/761640\/bj1090637.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T20:58:56Z","timestamp":1638219536000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/109\/4\/637\/6201\/Interchain-disulphide-bond-formation-in-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1968,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1968,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1090637","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1968,10,1]]}}}