{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T11:16:28Z","timestamp":1720610188612},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1969,7,1]]},"abstract":"<jats:p>The effect of various inhibitors on insulin release from pieces of rabbit pancreas incubated in vitro was studied. Insulin release was stimulated by glucose (3mg.\/ml.), leucine (5mm), tolbutamide (200\u03bcg.\/ml.), ouabain (10\u03bcm), a raised extracellular K+ concentration (60mm) and substitution of the Ca2+ content of the incubation medium by Ba2+ (2\u00b75mm). (a) Mannoheptulose (6mg.\/ml.) inhibited glucose-stimulated insulin release only. (b) Anoxia abolished or inhibited insulin release stimulated by glucose, leucine, tolbutamide and K+, but had little or no effect on release stimulated by ouabain or Ba2+. (c) 2,4-Dinitrophenol (0\u00b725mm) abolished or inhibited insulin release stimulated by glucose, ouabain or Ba2+. (d) Diazoxide (250\u03bcg.\/ml.) abolished or inhibited insulin release stimulated by glucose, leucine, tolbutamide, ouabain or Ba2+ (0\u00b725 or 1mm). Diazoxide had no effect on insulin release stimulated by Ba2+ (2\u00b75mm) and potentiated release stimulated by K+. (e) Adrenaline (1\u03bcm) abolished insulin release stimulated by glucose, leucine, tolbutamide, ouabain or Ba2+. K+-stimulated release was inhibited by adrenaline. (f) Tetrodotoxin (1\u03bcm) had no effect on insulin release stimulated by glucose, leucine, tolbutamide, ouabain, K+ or Ba2+. (g) Nupercaine (1mm) abolished insulin release stimulated by glucose or Ba2+.<\/jats:p>","DOI":"10.1042\/bj1130473","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:37:25Z","timestamp":1439501845000},"page":"473-479","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["The interaction of various inhibitors and stimuli of insulin release studied with rabbit pancreas <i>in vitro<\/i>"],"prefix":"10.1042","volume":"113","author":[{"given":"R. D. G.","family":"Milner","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. N.","family":"Hales","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/113\/3\/473\/763737\/bj1130473.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/113\/3\/473\/763737\/bj1130473.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T21:30:47Z","timestamp":1638221447000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/113\/3\/473\/19039\/The-interaction-of-various-inhibitors-and-stimuli"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1969,7,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1969,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1130473","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1969,7,1]]}}}