{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:58:43Z","timestamp":1762271923274},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1970,1,1]]},"abstract":"<jats:p>(1) Polysaccharides were isolated from bovine liver capsule by extraction with 2m-potassium chloride followed by precipitation from 0.8m-potassium chloride with cetylpyridinium chloride. Chondroitin sulphate was eliminated by digestion with hyaluronidase. The yield of heparin was approx. 40% of that obtained after extraction of the papain-digested tissue. (2) The macromolecular properties of the hyaluronidase-digested polysaccharide were studied by gel chromatography on Sephadex G-200 of the intact, as well as of the alkali-degraded, material. The results suggested the presence of single heparin chains in addition to a dermatan sulphate proteoglycan. (3) A purified heparin preparation was analysed for amino acids and neutral sugars. Xylose, galactose and serine were found in amounts corresponding to 0.1, 0.2, and 0.4 residue\/polysaccharide chain (mol.wt. 7400), respectively. It is suggested that the isolated material had been degraded by a polysaccharidase with endo-enzyme properties.<\/jats:p>","DOI":"10.1042\/bj1160027","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:26:00Z","timestamp":1439234760000},"page":"27-34","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Attempted isolation of a heparin proteoglycan from bovine liver capsule"],"prefix":"10.1042","volume":"116","author":[{"given":"U.","family":"Lindahl","sequence":"first","affiliation":[{"name":"Institute of Medical Chemistry, University of Uppsala, Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/116\/1\/27\/765774\/bj1160027.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/116\/1\/27\/765774\/bj1160027.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T18:58:36Z","timestamp":1637953116000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/116\/1\/27\/16785\/Attempted-isolation-of-a-heparin-proteoglycan-from"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1970,1,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1160027","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1970,1,1]]}}}