{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T21:35:41Z","timestamp":1771882541661,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1970,2,1]]},"abstract":"<jats:p>1. The thiol and disulphide contents of hen ovalbumin were investigated by p-chloromercuribenzoate titration, by determination of cysteic acid content after performic acid oxidation, by measurement of uptake of radioactive iodoacetic acid, and by assay of S-aminoethylcysteine after reaction with ethyleneimine. All results showed that ovalbumin had 6 half-cystine residues. Experiments with and without reducing agents demonstrated that there were 4 thiol groups and 1 disulphide bond. 2. A peptide containing equimolar amounts of S-carboxymethyl-cysteine, serine, valine and proline, but no lysine or arginine, was obtained by radioactive labelling of the cysteine residues with iodo[14C]acetic acid followed by electrophoretic and chromatographic separation of tryptic digests. It was concluded that the C-terminal sequence of ovalbumin is -Cys-Val-Ser-Pro. 3. The location of the disulphide bond was studied by using a double-labelling technique. It was shown that one end of the disulphide was located in this C-terminal peptide.<\/jats:p>","DOI":"10.1042\/bj1160555","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:26:00Z","timestamp":1439234760000},"page":"555-561","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":77,"title":["Thiol and disulphide contents of hen ovalbumin. <i>C<\/i>-Terminal sequence and location of disulphide bond"],"prefix":"10.1042","volume":"116","author":[{"given":"L. A.","family":"Fothergill","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Aberdeen, Marischal College, Aberdeen AB9 1AS, U.K."}]},{"given":"J. E.","family":"Fothergill","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Aberdeen, Marischal College, Aberdeen AB9 1AS, U.K."}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/116\/4\/555\/766742\/bj1160555.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/116\/4\/555\/766742\/bj1160555.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T19:43:38Z","timestamp":1637955818000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/116\/4\/555\/18769\/Thiol-and-disulphide-contents-of-hen-ovalbumin-C"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,2,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1970,2,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1160555","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1970,2,1]]}}}