{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:29:17Z","timestamp":1756992557017},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1970,7,1]]},"abstract":"<jats:p>Solutions of crystalline \u03b2-lactamase I and \u03b2-lactamase II, prepared by Kuwabara (1970), were examined in the ultracentrifuge and their sedimentation coefficients, diffusion coefficients, molecular weights and heterogeneity determined. Each sample was shown to consist of a major component comprising at least 97% of the material and a minor component of much higher molecular weight. The molecular weights of the major components were 27800 for \u03b2-lactamase I and 35600 for \u03b2-lactamase II. Emphasis is placed on a straightforward practical way of analysing the sedimentation-equilibrium results on mixtures of two macromolecular components rather than on a strict theoretical solution. Appendices describe the theory of systems at both chemical and sedimentation equilibrium and the procedure for calculating the combined distribution of two components.<\/jats:p>","DOI":"10.1042\/bj1180467","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:28:37Z","timestamp":1439234917000},"page":"467-474","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Sedimentation-equilibrium studies on the heterogeneity of two \u03b2-lactamases"],"prefix":"10.1042","volume":"118","author":[{"given":"P. H.","family":"Lloyd","sequence":"first","affiliation":[{"name":"Nuffield Department of Clinical Biochemistry, University of Oxford, Radcliffe Infirmary, Oxford OX2 6HE, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. R.","family":"Peacocke","sequence":"additional","affiliation":[{"name":"Nuffield Department of Clinical Biochemistry, University of Oxford, Radcliffe Infirmary, Oxford OX2 6HE, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/118\/3\/467\/768166\/bj1180467.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/118\/3\/467\/768166\/bj1180467.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T19:17:32Z","timestamp":1637954252000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/118\/3\/467\/17521\/Sedimentation-equilibrium-studies-on-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,7,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1970,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1180467","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1970,7,1]]}}}