{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:07:05Z","timestamp":1762067225075},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1971,3,1]]},"abstract":"<jats:p>1. Methanol or formate can replace serine or glycine as supplements for growth on succinate of the auxotrophic mutants 20S and 82G of Pseudomonas AM1, showing that the organism can synthesize glycine and serine in net fashion from C1 units. 2. Double mutants of Pseudomonas 20S and 82G have been prepared (20ST-1 and 82GT-1) that are unable to grow on succinate+1mm-glyoxylate, succinate+2mm-methanol or methanol alone. 3. Mutants 20ST-1 and 82GT-1 lacked serine\u2013glyoxylate aminotransferase activity, and revertants to the phenotype of 20S and 82G regained serine\u2013glyoxylate aminotransferase activity. A total revertant of 82GT-1 to wild-type phenotype regained activities of serine hydroxymethyltransferase and serine\u2013glyoxylate aminotransferase. 4. The activity of serine\u2013glyoxylate aminotransferase in methanol-grown Pseudomonas AM1 is eightfold higher than in the succinate-grown organism. 5. The combined results show that in Pseudomonas AM1 serine\u2013glyoxylate aminotransferase is necessary for growth on C1 compounds and is involved in the conversion of methanol into glycine via glyoxylate. 6. It is suggested that the phosphorylated pathway of serine biosynthesis from phosphoglycerate replenishes the supply of \u03b1-amino groups necessary for the flow of glyoxylate through the main assimilatory pathway during growth on C1 compounds.<\/jats:p>","DOI":"10.1042\/bj1210763","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:32:13Z","timestamp":1439235133000},"page":"763-769","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Aspects of glycine and serine biosynthesis during growth of <i>Pseudomonas<\/i> AM1 on C1 compounds"],"prefix":"10.1042","volume":"121","author":[{"given":"W.","family":"Harder","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Sheffield, Sheffield S10 2TN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"Quayle","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Sheffield, Sheffield S10 2TN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/121\/5\/763\/770337\/bj1210763.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/121\/5\/763\/770337\/bj1210763.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T19:09:17Z","timestamp":1637953757000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/121\/5\/763\/17181\/Aspects-of-glycine-and-serine-biosynthesis-during"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,3,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1971,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1210763","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1971,3,1]]}}}