{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T21:13:17Z","timestamp":1749071597855},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1971,5,1]]},"abstract":"<jats:p>1. A pseudomonad capable of utilizing 4-chlorophenoxyacetate (CPA) as sole source of organic carbon was isolated from soil. 2. The organism was grown in liquid culture and the following compounds were isolated and identified in culture extracts: 4-chloro-2-hydroxyphenoxyacetate, 4-chlorocatechol, \u03b2-chloromuconate probably the cis\u2013trans isomer and \u03b3-carboxymethylene-\u0394\u03b1\u03b2-butenolide. 3. Cells grown on 4-chlorophenoxyacetate were able to metabolize 4-chloro-2-hydroxyphenoxyacetate, 4-chlorocatechol and \u03b3-carboxymethylene-\u0394\u03b1\u03b2-butenolide without a lag period. They were not adapted to 4-chlorophenol, or to either culture isolated or synthetic \u03b2-chloromuconate, possibly because of stereospecificity towards the cis\u2013cis isomer. 4. On the basis of isolation and induction evidence, the following metabolic pathway is proposed for the breakdown of 4-chlorophenoxyacetate by this organism: 4-chlorophenoxyacetate \u2192 4-chloro-2-hydroxyphenoxyacetate \u2192 4-chlorocatechol \u2192 cis\u2013cis-\u03b2-chloromuconate \u2192 \u03b3-carboxymethylene-\u0394\u03b1\u03b2-butenolide \u2192 maleylacetate and fumarylacetate \u2192 fumarate and acetate.<\/jats:p>","DOI":"10.1042\/bj1220509","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:37:15Z","timestamp":1439501835000},"page":"509-517","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":79,"title":["Bacterial metabolism of 4-chlorophenoxyacetate"],"prefix":"10.1042","volume":"122","author":[{"given":"W. C.","family":"Evans","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Soil Science, University College of North Wales, Bangor, Caerns., U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. S. W.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Soil Science, University College of North Wales, Bangor, Caerns., U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Moss","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Soil Science, University College of North Wales, Bangor, Caerns., U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. N.","family":"Fernley","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Soil Science, University College of North Wales, Bangor, Caerns., U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/122\/4\/509\/770595\/bj1220509.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/122\/4\/509\/770595\/bj1220509.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T19:24:01Z","timestamp":1637954641000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/122\/4\/509\/17674\/Bacterial-metabolism-of-4-chlorophenoxyacetate"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,5,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1971,5,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1220509","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1971,5,1]]}}}