{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T21:41:49Z","timestamp":1700689309152},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1971,5,1]]},"abstract":"<jats:p>1. A cell-free system, prepared from Pseudomonas N.C.I.B. 9340 grown on 4-chloro-2-methylphenoxyacetate (MCPA) was shown to catalyse the reaction sequence: 5-chloro-3-methylcatechol \u2192 cis\u2013cis-\u03b3-chloro-\u03b1-methylmuconate \u2192 \u03b3-carboxymethylene-\u03b1-methyl-\u0394\u03b1\u03b2-butenolide \u2192 \u03b3-hydroxy-\u03b1-methylmuconate. 2. The activity of the three enzymes involved in these reactions was completely resolved and the lactonizing and delactonizing enzymes were separated. 3. This part of the metabolic pathway of 4-chloro-2-methylphenoxyacetate is thus confirmed for this bacterium. 4. The ring-fission oxygenase required Fe2+ or Fe3+ and reduced glutathione for activity; the lactonizing enzyme is stimulated by Mn2+, Mg2+, Co2+ and Fe2+; no cofactor requirement could be demonstrated for the delactonizing enzyme. 5. cis\u2013cis-\u03b3-Chloro-\u03b1-methylmuconic acid was isolated and found to be somewhat unstable, readily lactonizing to \u03b3-carboxymethylene-\u03b1-methyl-\u0394\u03b1\u03b2-butenolide. 6. Enzymically the lactonization appears to be a single-step dehydrochlorinase reaction.<\/jats:p>","DOI":"10.1042\/bj1220533","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:37:15Z","timestamp":1439501835000},"page":"533-542","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Metabolism of 4-chloro-2-methylphenoxyacetate by a soil pseudomonad. Ring-fission, lactonizing and delactonizing enzymes"],"prefix":"10.1042","volume":"122","author":[{"given":"J. K.","family":"Gaunt","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Soil Science, University College of North Wales, Bangor, Caerns., U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. C.","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Soil Science, University College of North Wales, Bangor, Caerns., U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/122\/4\/533\/770601\/bj1220533.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/122\/4\/533\/770601\/bj1220533.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T19:24:16Z","timestamp":1637954656000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/122\/4\/533\/17679\/Metabolism-of-4-chloro-2-methylphenoxyacetate-by-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,5,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1971,5,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1220533","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1971,5,1]]}}}