{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T08:32:38Z","timestamp":1768725158404,"version":"3.49.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1971,5,1]]},"abstract":"<jats:p>1. Two Pseudomonas strains isolated from soil metabolized 2,4-dichlorophenoxyacetate (2,4-D) as sole carbon source in mineral salts liquid medium. 2. 2,4-Dichlorophenoxyacetate cultures of Pseudomonas I (Smith, 1954) contained 2,4-dichlorophenol, 2-chlorophenol, 3,5-dichlorocatechol and \u03b1-chloromuconate, the last as a major metabolite. 3. Dechlorination at the 4(p)-position of the aromatic ring must therefore take place at some stages before ring fission. 4. Pseudomonas N.C.I.B. 9340 (Gaunt, 1962) cultures metabolizing 2,4-dichlorophenoxyacetate contained 2,4-dichloro-6-hydroxyphenoxyacetate, 2,4-dichlorophenol, 3,5-dichlorocatechol and an unstable compound, probably \u03b1\u03b3-dichloromuconate. 5. Cell-free extracts of the latter organism grown in 2,4-dichlorophenoxyacetate cultures contained an oxygenase that converted 3,5-dichlorocatechol into \u03b1\u03b3-dichloromuconate, a chlorolactonase that in the presence of Mn2+ ions converted the dichloromuconate into \u03b3-carboxymethylene-\u03b1-chloro-\u0394\u03b1\u03b2-butenolide, and a delactonizing enzyme that gave \u03b1-chloromaleylacetate from this lactone. 6. Pathways of metabolism of 2,4-dichlorophenoxyacetate are discussed.<\/jats:p>","DOI":"10.1042\/bj1220543","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:37:15Z","timestamp":1439501835000},"page":"543-551","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":156,"title":["Bacterial metabolism of 2,4-dichlorophenoxyacetate"],"prefix":"10.1042","volume":"122","author":[{"given":"W. C.","family":"Evans","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Soil Science, University College of North Wales, Bangor, Caerns., U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. S. W.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Soil Science, University College of North Wales, Bangor, Caerns., U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. N.","family":"Fernley","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Soil Science, University College of North Wales, Bangor, Caerns., U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. I.","family":"Davies","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Soil Science, University College of North Wales, Bangor, Caerns., U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/122\/4\/543\/770641\/bj1220543.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/122\/4\/543\/770641\/bj1220543.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T19:25:04Z","timestamp":1637954704000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/122\/4\/543\/17698\/Bacterial-metabolism-of-2-4-dichlorophenoxyacetate"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,5,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1971,5,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1220543","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1971,5,1]]}}}