{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T08:28:52Z","timestamp":1759048132098},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1971,11,1]]},"abstract":"<jats:p>Incorporation of radioactive fucose into the immunoglobulin G1 myeloma protein secreted by mouse plasma-cell tumour MOPC 21 is stereospecific for the l-isomer. Heavy chains of the secreted form of the myeloma protein carry 90% of the label in fucose residues of their carbohydrate moieties. A small but significant amount of the intracellular immunoglobulin G1 of the mouse plasma-cell tumour MOPC 21 appears to be labelled. Serum in the incubation medium supplies low-molecular-weight diffusible substances necessary to maintain continuous secretion of fucose-labelled myeloma protein beyond 2\u20133h, and of leucine-labelled myeloma protein beyond 6\u20138h. In medium containing extensively dialysed serum the secretion of leucine- and fucose-labelled myeloma protein can be restored by the addition of 250\u03bcm-d-mannose, 250\u03bcm-d-galactose and 250\u03bcm-glucosamine. Synthesis and secretion appear to be facilitated in the presence of these sugars, although secretion of myeloma protein devoid of terminal fucose residues is possible for a limited time-period.<\/jats:p>","DOI":"10.1042\/bj1250241","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:39:23Z","timestamp":1439501963000},"page":"241-247","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Biosynthesis of the carbohydrate portion of immunoglobulins. Incorporation of radioactive fucose into immunoglobulin G1 synthesized and secreted by mouse plasma-cell tumour MOPC 21"],"prefix":"10.1042","volume":"125","author":[{"given":"Fritz","family":"Melchers","sequence":"first","affiliation":[{"name":"Max Planck Institut f\u00fcr Molekulare Genetik, Abteilung Trautner, 1 Berlin 33, West Germany, and Department of Genetics, Stanford University Medical School, Stanford, Calif. 94305, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/125\/1\/241\/773700\/bj1250241.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/125\/1\/241\/773700\/bj1250241.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T19:58:20Z","timestamp":1637956700000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/125\/1\/241\/89033\/Biosynthesis-of-the-carbohydrate-portion-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,11,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1971,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1250241","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1971,11,1]]}}}