{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:13:15Z","timestamp":1762161195124},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1971,11,1]]},"abstract":"<jats:p>1. A system has been developed for the specific transfer of [3H]dihydrotestosterone\u2013receptor complexes into prostatic chromatin in vitro. 2. Under optimum conditions the overall transfer of [3H]dihydrotestosterone into purified chromatin in this reconstituted system is entirely consistent with the results obtained in whole tissue both in vivo and in vitro. 3. The transfer of [3H]dihydrotestosterone into chromatin is tissue-specific and maximal into chromatin isolated from androgen-dependent tissues. 4. The tissue specificity is maintained at two levels: first, in the presence of specific cytoplasmic androgen-receptor proteins; secondly, by the nature and composition of the chromatin itself. 5. Evidence is presented that androgenic steroids in vivo may maintain the tissue-specific nature of chromatin in androgen-dependent tissues by the selective induction of nuclear protein synthesis. 6. The relevance of these findings to the mechanism of action of androgenic steroids is discussed.<\/jats:p>","DOI":"10.1042\/bj1250285","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:39:23Z","timestamp":1439501963000},"page":"285-295","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":137,"title":["A reconstituted cell-free system for the specific transfer of steroid\u2013receptor complexes into nuclear chromatin isolated from rat ventral prostate gland"],"prefix":"10.1042","volume":"125","author":[{"given":"W. I. P.","family":"Mainwaring","sequence":"first","affiliation":[{"name":"Endocrinology Group, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brenda M.","family":"Peterken","sequence":"additional","affiliation":[{"name":"Endocrinology Group, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/125\/1\/285\/773662\/bj1250285.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/125\/1\/285\/773662\/bj1250285.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T19:57:39Z","timestamp":1637956659000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/125\/1\/285\/88964\/A-reconstituted-cell-free-system-for-the-specific"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,11,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1971,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1250285","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1971,11,1]]}}}