{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T15:24:26Z","timestamp":1782919466687,"version":"3.54.5"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1971,11,1]]},"abstract":"<jats:p>1. The egg-shell of the oriental garden cricket, Gryllus mitratus, contained at least two different types of structural protein in an approximate ratio of 5:1. The major fraction was extracted in a solvent containing dithiothreitol, EDTA and 8m-urea, and was purified to apparent homogeneity as judged by free-boundary electrophoresis and ultracentrifugation. This was designated SH-fraction and its S-carboxymethyl derivative (CM-fraction) was also prepared. The minor fraction, insoluble in the solvent, was designated insoluble residue. 2. The major fraction was a phosphoprotein, rich in serine (29.8mol% of the total amino acids) and phosphate (nearly equimolar to serine), and O-phosphoserine was identified in its partial acid hydrolysate. The content of cystine was rather low (0.9mol%) in spite of the importance of this amino acid residue in the native form of the protein. The insoluble residue contained only a small amount of phosphorus, and its amino acid composition was clearly different from the major fraction. 3. CM-fraction, a fibrous protein with an average molecular weight of 57500, behaved as a typical polyanion owing to the high content of phosphate. SH-fraction and CM-fraction were precipitable from their aqueous solutions by the addition of bivalent metal cations, and the precipitation of CM-fraction by Ca2+ and Mg2+ was studied in detail. 4. When SH-fraction was exposed to air, intermolecular disulphide linkages were formed, yielding a net-like gel that changed its volume with changes in Ca2+, Mg2+ and Na+. 5. The possible role of this protein fraction in maintaining the integrity of the egg-shell, and a comparison of its composition and properties with other egg-shell proteins and other phosphoproteins, are discussed.<\/jats:p>","DOI":"10.1042\/bj1250495","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:39:23Z","timestamp":1439501963000},"page":"495-505","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Structural proteins in the egg-shell of the oriental garden cricket, <i>Gryllus mitratus<\/i>"],"prefix":"10.1042","volume":"125","author":[{"given":"Hiroya","family":"Kawasaki","sequence":"first","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, Iwate Medical College, Morioka 020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, Iwate Medical College, Morioka 020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, School of Dentistry, Iwate Medical College, Morioka 020, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/125\/2\/495\/774056\/bj1250495.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/125\/2\/495\/774056\/bj1250495.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T15:44:48Z","timestamp":1637941488000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/125\/2\/495\/8513\/Structural-proteins-in-the-egg-shell-of-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,11,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1971,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1250495","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1971,11,1]]}}}