{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,20]],"date-time":"2025-12-20T21:47:11Z","timestamp":1766267231617},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1971,12,1]]},"abstract":"<jats:p>Bacteriophage \u03bc2 is inactivated by both mono- and di-functional sulphur mustards at relatively low extents of alkylation. No degradation of alkylated RNA was detected. Cross-linking of RNA to protein was observed with the difunctional agent, but this reaction was only a minor contribution to the inactivation. Analyses of the reaction products in bacteriophage RNA showed that, at the mean lethal doses, more than one mono-alkylation of guanine had occurred but the sum total of other types of RNA alkylation was close to a single event. The results therefore suggest that inactivation results from the mono-alkylation of adenine or cytosine. In experiments with the difunctional agent cross-linking of RNA bases or of RNA to protein also prevented replication, the existence of these reactions accounting for the greater sensitivity of the bacteriophage to this agent.<\/jats:p>","DOI":"10.1042\/bj1250829","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:39:23Z","timestamp":1439501963000},"page":"829-840","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The action of mono- and di-functional sulphur mustards on the ribonucleic acid-containing bacteriophage \u03bc2"],"prefix":"10.1042","volume":"125","author":[{"given":"K. V.","family":"Shooter","sequence":"first","affiliation":[{"name":"Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, London S.W.3. U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. A.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, London S.W.3. U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. D.","family":"Lawley","sequence":"additional","affiliation":[{"name":"Chester Beatty Research Institute, Institute of Cancer Research, Royal Cancer Hospital, London S.W.3. U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/125\/3\/829\/774066\/bj1250829.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/125\/3\/829\/774066\/bj1250829.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T19:51:32Z","timestamp":1637956292000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/125\/3\/829\/19949\/The-action-of-mono-and-di-functional-sulphur"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,12,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1971,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1250829","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1971,12,1]]}}}