{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:51:17Z","timestamp":1775065877362,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1972,9,1]]},"abstract":"<jats:p>A fractionation procedure is described which allows the isolation of three major human erythrocyte membrane proteins. Their isolation involves three sequential extraction procedures followed by gel filtration in 1% sodium dodecyl sulphate and preparative gel electrophoresis. All three proteins can be isolated from a single preparation. One of the proteins is the erythrocyte sialoglycoprotein, for which no C- or N-terminal residues were found. The other two proteins, which have not previously been isolated, have subunit molecular weights of 74000 and 93000 and contain 9 and 7% carbohydrate respectively. These glycoproteins have blocked N-terminal residues and show similarities in their chemical properties. Preparations derived from blood-group O erythrocytes contain no N-acetylgalactosamine, but similar preparations from blood-group A erythrocytes do contain this sugar. These three proteins cannot easily be solubilized by gentle aqueous procedures and represent about half of the erythrocyte \u2018ghost\u2019 protein. They carry a large proportion of the cell-surface carbohydrate.<\/jats:p>","DOI":"10.1042\/bj1290333","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:46:11Z","timestamp":1439235971000},"page":"333-347","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":119,"title":["Separation and some properties of the major proteins of the human erythrocyte membrane"],"prefix":"10.1042","volume":"129","author":[{"given":"M. J. A.","family":"Tanner","sequence":"first","affiliation":[{"name":"Molecular Enzymology Laboratory, Department of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. H.","family":"Boxer","sequence":"additional","affiliation":[{"name":"Molecular Enzymology Laboratory, Department of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/129\/2\/333\/778684\/bj1290333.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/129\/2\/333\/778684\/bj1290333.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T15:49:03Z","timestamp":1637941743000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/129\/2\/333\/8668\/Separation-and-some-properties-of-the-major"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,9,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1972,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1290333","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1972,9,1]]}}}