{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:35:34Z","timestamp":1773657334493,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"4","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1972,10,1]]},"abstract":"<jats:p>The purification of wheat-germ agglutinin by precipitation with ammonium sulphate and by chromatography on Sephadex G-75, Sepharose\u2013ovomucoid and CM-cellulose is described. This procedure gave agglutinin preparations which were homogeneous on polyacrylamide gels under a variety of conditions. Purified wheat-germ agglutinin formed colourless solutions and was relatively insoluble at neutral pH; maximum solubility in 1mm-tris\u2013HCl buffer, pH7.4, was approx. 1mg\/ml. The agglutinin was a glycoprotein containing a single polypeptide chain with an approximate molecular weight of 23000. The N-terminus of the oxidized agglutinin was cysteic acid and the C-terminus was glycine. The amino acid composition showed that the protein was extremely rich in cysteine and cystine; there were 15\u201317 free SH groups\/mol. The absorption maximum for the protein was at 272nm and the molar extinction coefficient at 280nm was 1.09\u00d7105 litre\u00b7mol-1\u00b7cm-1. Equilibrium dialysis indicated that there was only one binding site per molecule for N-acetylglucosamine.<\/jats:p>","DOI":"10.1042\/bj1290847","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:46:11Z","timestamp":1439235971000},"page":"847-856","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":105,"title":["The purification and characterization of wheat-germ agglutinin"],"prefix":"10.1042","volume":"129","author":[{"given":"Dreania","family":"LeVine","sequence":"first","affiliation":[{"name":"Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y. 11724, U.S.A."}]},{"given":"Michael J.","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y. 11724, U.S.A."}]},{"given":"Peter J.","family":"Greenaway","sequence":"additional","affiliation":[{"name":"Cold Spring Harbor Laboratory, Cold Spring Harbor, N. Y. 11724, U.S.A."}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/129\/4\/847\/778942\/bj1290847.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/129\/4\/847\/778942\/bj1290847.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T15:01:15Z","timestamp":1637938875000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/129\/4\/847\/7646\/The-purification-and-characterization-of-wheat"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,10,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1972,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1290847","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1972,10,1]]}}}