{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,4]],"date-time":"2023-11-04T18:09:07Z","timestamp":1699121347111},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1972,10,1]]},"abstract":"<jats:p>1. Analysis of transport of d-galactose was complicated by metabolism of the compound but appeared to have two components: a substrate-saturable component and a diffusion component. At low substrate concentration (&amp;lt;1mm) active transport was observed. Accumulation of galactose was largely independent of Na+concentration. The apparent Km for this component was 0.2mm. At substrate concentrations above 1mm the active transport system appeared saturated and further increases in substrate concentration resulted in a linear increase in the rate of galactose accumulation, but no concentration gradient was formed. 2. d-[1-14C]Galactose (2mm) was metabolized to14CO2 by rat kidney-cortex slices incubated at 37\u00b0C, at the rate of 68nmol\/h per 100mg of tissue. 3. Intracellular components from such incubations were separated into a neutral fraction, the only major labelled component being galactose, and a phosphorylated fraction. 4. Phosphorylated metabolites found in galactose-incubated slices increased with increasing substrate concentration and achieved a limiting value of 0.42mm after 60min of incubation. 5. Galactose uptake was inhibited by anaerobiosis, dinitrophenol and phlorrhizin. 6. Methyl \u03b1-d-glucoside and d-glucose partially inhibited galactose uptake only at ratios of 100:1. 7. The presence of pyruvate did not decrease galactose metabolism although it did decrease production of14CO2 from [1-14C]galactose. Gluconeogenesis occurred in the presence of pyruvate and14C from galactose was found in glucose. 8. Rat kidney-cortex slices metabolized 2mm-[1-14C]galactonate to14CO2 at a rate of 20nmol\/h per 100mg of tissue.<\/jats:p>","DOI":"10.1042\/bj1291109","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:46:11Z","timestamp":1439235971000},"page":"1109-1118","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Transport and metabolism of galactose in rat kidney cortex"],"prefix":"10.1042","volume":"129","author":[{"given":"Pamela D.","family":"McNamara","sequence":"first","affiliation":[{"name":"Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, and the Departments of Pediatrics and Medicine, Medical School of the University of Philadelphia, Philadelphia, Pa. 19146, U.S.A."}]},{"given":"Stanton","family":"Segal","sequence":"additional","affiliation":[{"name":"Division of Biochemical Development and Molecular Diseases, Children's Hospital of Philadelphia, and the Departments of Pediatrics and Medicine, Medical School of the University of Philadelphia, Philadelphia, Pa. 19146, U.S.A."}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/129\/5\/1109\/778966\/bj1291109.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/129\/5\/1109\/778966\/bj1291109.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T15:04:59Z","timestamp":1637939099000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/129\/5\/1109\/7735\/Transport-and-metabolism-of-galactose-in-rat"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,10,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1972,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1291109","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1972,10,1]]}}}