{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:31:27Z","timestamp":1756992687527},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1973,5,1]]},"abstract":"<jats:p>A rapid procedure involving DNA\u2013cellulose chromatography followed either by sedimentation in a high-salt glycerol gradient or by gel filtration is described for the complete purification of Escherichia coli DNA-dependent RNA polymerase.<\/jats:p>","DOI":"10.1042\/bj1330201","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:49:22Z","timestamp":1439236162000},"page":"201-203","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["A procedure for the rapid purification of <i>Escherichia coli<\/i> deoxyribonucleic acid-dependent ribonucleic acid polymerase (<i>Short Communication<\/i>)"],"prefix":"10.1042","volume":"133","author":[{"given":"Peter","family":"Humphries","sequence":"first","affiliation":[{"name":"Department of Genetics, Trinity College, Dublin 2, Irish Republic"}]},{"given":"David J.","family":"McConnell","sequence":"additional","affiliation":[{"name":"Department of Genetics, Trinity College, Dublin 2, Irish Republic"}]},{"given":"Robert L.","family":"Gordon","sequence":"additional","affiliation":[{"name":"Department of Genetics, Trinity College, Dublin 2, Irish Republic"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/133\/1\/201\/560955\/bj1330201.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/133\/1\/201\/560955\/bj1330201.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T14:59:33Z","timestamp":1637938773000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/133\/1\/201\/7589\/A-procedure-for-the-rapid-purification-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,5,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1973,5,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1330201","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1973,5,1]]}}}