{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:10:06Z","timestamp":1767625806061},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1973,5,15]]},"abstract":"<jats:p>Chloroplasts isolated from young spinach leaves incorporate [3H]uridine into RNA. This incorporation shows an absolute requirement for light and does not occur in lysed chloroplasts. Fractionation by polyacrylamide-gel electrophoresis of the RNA synthesized in vitro reveals a major discrete product of molecular weight 2.7\u00d7106 and two minor products of molecular weight 1.2\u00d7106 and 0.47\u00d7106. These discrete products are super-imposed on a background of polydisperse RNA. The incorporation of 32Pi into chloroplast rRNA species (mol.wt. 1.05\u00d7106 and 0.56\u00d7106) in excised spinach leaves proceeds after a distinct lag period compared with the incorporation into cytoplasmic rRNA species (mol.wt. 1.34\u00d7106 and 0.7\u00d7106). Incorporation of 32Pi into chloroplast RNA species of molecular weight 2.7\u00d7106, 1.2\u00d7106, 0.65\u00d7106 and 0.47\u00d7106 proceeds without such a time-lag. The kinetics of labelling of the individual RNA components is consistent with the rapidly labelled RNA species of molecular weight 1.2\u00d7106 and 0.65\u00d7106 being precursors to the more slowly labelled rRNA species of molecular weight 1.05\u00d7106 and 0.56\u00d7106 respectively.<\/jats:p>","DOI":"10.1042\/bj1340249","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:50:06Z","timestamp":1439236206000},"page":"249-262","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Ribonucleic acid synthesis in chloroplasts"],"prefix":"10.1042","volume":"134","author":[{"given":"M. R.","family":"Hartley","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Ellis","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/134\/1\/249\/780457\/bj1340249.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/134\/1\/249\/780457\/bj1340249.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T15:21:12Z","timestamp":1637940072000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/134\/1\/249\/8115\/Ribonucleic-acid-synthesis-in-chloroplasts"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,5,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1973,5,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1340249","relation":{},"ISSN":["0306-3283"],"issn-type":[{"value":"0306-3283","type":"print"}],"subject":[],"published":{"date-parts":[[1973,5,15]]}}}