{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:43:28Z","timestamp":1758278608011},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1974,3,1]]},"abstract":"<jats:p>The distribution of protein and blood-group-antigen activity obtained after butanol extraction of erythrocyte `ghosts' under various conditions is described. Butanol extraction under low-ionic strength conditions results in the recovery of membrane protein in high yield in the aqueous phase. Blood-group-A activity is found in both the aqueous and butanol phases, whereas blood-group-P activity is confined to the butanol phase and blood-group-I and blood-group-MN activity are restricted to the aqueous phase. Much lower yields of protein are obtained in the aqueous phase when high-ionic-strength conditions are used. An appreciable amount of material is precipitated at the interface. Under these conditions blood-group-P activity is found only in the butanol phase, blood group-A activity in the butanol phase and interface material and only blood-group-MN activity in the aqueous phase. In contrast with previous reports no correlation could be demonstrated between the secretor status of the donors and the presence of blood-group-A activity in the aqueous phase after butanol extraction under any of the extraction conditions used. By using butanol extraction under high-ionic-strength conditions it is possible to isolate the blood-group-MN-active sialoglycoprotein in high yield from erythrocyte `ghosts' by a simple procedure.<\/jats:p>","DOI":"10.1042\/bj1380381","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:52:40Z","timestamp":1439236360000},"page":"381-386","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["The distribution of blood-group antigens on butanol extraction of human erythrocyte \u2018ghosts\u2019"],"prefix":"10.1042","volume":"138","author":[{"given":"D. J.","family":"Anstee","sequence":"first","affiliation":[{"name":"South West Regional Blood Transfusion Centre, Southmead, Bristol BS10 5ND, U.K., and Department of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K."}]},{"given":"M. J. A.","family":"Tanner","sequence":"additional","affiliation":[{"name":"South West Regional Blood Transfusion Centre, Southmead, Bristol BS10 5ND, U.K., and Department of Biochemistry, University of Bristol, Bristol BS8 1TD, U.K."}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/138\/3\/381\/561947\/bj1380381.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/138\/3\/381\/561947\/bj1380381.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T13:27:38Z","timestamp":1637933258000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/138\/3\/381\/4799\/The-distribution-of-blood-group-antigens-on"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1974,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1380381","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1974,3,1]]}}}