{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:31:34Z","timestamp":1762079494960},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1974,6,1]]},"abstract":"<jats:p>Rates of glucose oxidation and insulin release in response to a wide range of glucose concentrations were studied in short-term experiments in isolated mouse pancreatic islets maintained in tissue culture for 6 days at either a physiological glucose concentration (6.7mm) or at a high glucose concentration (28mm). The curves relating glucose oxidation or insulin release to the extracellular glucose concentration obtained with islets cultured in 6.7mm-glucose displayed a sigmoid shape similar to that observed for freshly isolated non-cultured islets. By contrast islets that had been cultured in 28mm-glucose showed a linear relationship between the rate of glucose oxidation and the extracellular glucose concentration up to about 8mm-glucose. The maximal oxidative rate was twice that of the non-cultured islets and the glucose concentration associated with the half-maximal rate considerably decreased. In islets cultured at 28mm-glucose there was only a small increase in the insulin release in response to glucose, probably due to a depletion of stored insulin in those B cells that had been cultured in a high-glucose medium. It is concluded that exposure of B cells for 6 days to a glucose concentration comparable with that found in diabetic individuals causes adaptive metabolic alterations rather than degeneration of these cells.<\/jats:p>","DOI":"10.1042\/bj1400377","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:53:43Z","timestamp":1439236423000},"page":"377-382","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Long-term effects of glucose on insulin release and glucose oxidation by mouse pancreatic islets maintained in tissue culture"],"prefix":"10.1042","volume":"140","author":[{"given":"Arne","family":"Andersson","sequence":"first","affiliation":[{"name":"Department of Histology, University of Uppsala, Uppsala, Sweden"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/140\/3\/377\/562262\/bj1400377.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/140\/3\/377\/562262\/bj1400377.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T16:11:45Z","timestamp":1637943105000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/140\/3\/377\/9286\/Long-term-effects-of-glucose-on-insulin-release"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,6,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1974,6,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1400377","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1974,6,1]]}}}