{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:28:51Z","timestamp":1773656931258,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1975,2,1]]},"abstract":"<jats:p>Rabbit immunoglobulin gamma (IgG) was digested with plasmin after being left for 15 min at pH2.5, 30 degrees C followed by a rapid increase in the pH to 7. The fragment antigen and complement binding (Facb) was isolated and characterized chemically and biologically. Sequence studies showed that the C-terminal quarter of the heavy chain had been removed, the split occurring at a lysine-alanine bond in the sequence Thr-Ile-Ser-Lys-Ala-Arg. The fragment Facb retained the capacity to precipitate with antigen and the precipitate caused activation of the first component of complement of the same order as that of acid-treated IgG. Both Facb and acid-treated IgG showed a fall in complement fixation relative to the native molecule of 30-40%.<\/jats:p>","DOI":"10.1042\/bj1450177","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T19:56:36Z","timestamp":1439236596000},"page":"177-183","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":100,"title":["Characterization of a plasmin-digest fragment of rabbit immunoglobulin gamma that binds antigen and complement"],"prefix":"10.1042","volume":"145","author":[{"given":"M","family":"Colomb","sequence":"first","affiliation":[{"name":"M.R.C. Immunochemistry Unit, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, U.K."}]},{"given":"R R","family":"Porter","sequence":"additional","affiliation":[{"name":"M.R.C. Immunochemistry Unit, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, U.K."}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/145\/2\/177\/564120\/bj1450177.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/145\/2\/177\/564120\/bj1450177.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T16:50:36Z","timestamp":1637945436000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/145\/2\/177\/9889\/Characterization-of-a-plasmin-digest-fragment-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1975,2,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1450177","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1975,2,1]]}}}