{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:07:02Z","timestamp":1762067222857},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1977,10,15]]},"abstract":"<jats:p>1. Confluent human skin fibroblasts maintained in a chemically defined medium incorporate l-[1-3H]fucose in a linear manner with time into non-diffusible macromolecules for up to 48h. Chromatographic analysis demonstrated that virtually all the macromolecule-associated3H was present as [3H]fucose. 2. Equilibrium CsCl-density-gradient centrifugation established that [3H]fucose-labelled macromolecules released into the medium were predominantly glycoproteins. Confirmation of this finding was provided by molecular-size analyses of the [3H]fucose-labelled material before and after trypsin digestion. 3. The [3H]fucose-labelled glycoproteins released into fibroblast culture medium were analysed by gel-filtration chromatography and sodium dodecyl sulphate\/polyacrylamide-gel electrophoresis. These techniques demonstrated that the major fucosylated glycoprotein had an apparent mol.wt. of 230000\u2013250000; several minor labelled species were also detected. 4. Dual-labelling experiments with [3H]fucose and14C-labelled amino acids indicated that the major fucosylated glycoprotein was synthesized de novo by cultured fibroblasts. The non-collagenous nature of this glycoprotein was established by three independent methods. 5. Gel-filtration analysis before and after reduction with dithiothreitol showed that the major glycoprotein occurs as a disulphide-bonded dimer when analysed under denaturing conditions. Further experiments demonstrated that this glycoprotein was the predominant labelled species released into the medium when fibroblasts were incubated with [35S]cysteine. 6. The relationship between the major fucosylated glycoprotein and a glycoprotein, or group of glycoproteins, variously known as fibronectin, LETS protein, cell-surface protein etc., is discussed.<\/jats:p>","DOI":"10.1042\/bj1680091","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:09:18Z","timestamp":1439237358000},"page":"91-103","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Biosynthesis and release of glycoproteins by human skin fibroblasts in culture"],"prefix":"10.1042","volume":"168","author":[{"given":"Christopher H. J.","family":"Sear","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, The Medical School, University of Manchester, Oxford Road, Manchester M13 9PT, U.K."}]},{"given":"Michael E.","family":"Grant","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, The Medical School, University of Manchester, Oxford Road, Manchester M13 9PT, U.K."}]},{"given":"David S.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, The Medical School, University of Manchester, Oxford Road, Manchester M13 9PT, U.K."}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/168\/1\/91\/568753\/bj1680091.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/168\/1\/91\/568753\/bj1680091.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T17:35:14Z","timestamp":1637948114000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/168\/1\/91\/11257\/Biosynthesis-and-release-of-glycoproteins-by-human"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,10,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1977,10,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1680091","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1977,10,15]]}}}