{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T14:00:44Z","timestamp":1770991244400,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1979,7,15]]},"abstract":"<jats:p>The N-oxidation of NN-dimethylaniline was studied by using a reconstituted rabbit liver microsomal enzyme system consisting of highly purified cytochrome P-448, NADPH-cytochrome c reductase and lipid factor. Both cytochrome P-448 and NADPH-cytochrome c reductase were required for optimum N-oxygenating activity; the catalytic capacity of the reductase fraction for supporting N-oxide formation varied with the isolation procedure applied. Addition of microsomal lipids to the assay media stimulated N-oxidation of the arylamine. N-Oxide formation appeared to be not generally controlled by electron transfer from cytochrome b5 to cytochrome P-448. The present work confirms that cytochrome P-448 can mediate about 44% of the rabbit liver microsomal N-oxidation of NN-dimethylaniline, thus reinforcing the existence of at least two distinct tertiary amine N-oxidases, i.e. haemoprotein and flavoprotein oxidase, in liver microsomal fractions.<\/jats:p>","DOI":"10.1042\/bj1820109","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:18:33Z","timestamp":1439237913000},"page":"109-116","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Studies on the mechanism of hepatic microsomal <i>N<\/i>-oxide formation. <i>N<\/i>-oxidation of <i>NN<\/i>-dimethylaniline by a reconstituted rabbit liver microsomal cytochrome <i>P<\/i>-448 enzyme system"],"prefix":"10.1042","volume":"182","author":[{"given":"P","family":"Hlavica","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"H\u00fclsmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/182\/1\/109\/572854\/bj1820109.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/182\/1\/109\/572854\/bj1820109.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T13:11:15Z","timestamp":1637932275000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/182\/1\/109\/4406\/Studies-on-the-mechanism-of-hepatic-microsomal-N"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,7,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1979,7,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1820109","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1979,7,15]]}}}