{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,31]],"date-time":"2022-03-31T12:21:25Z","timestamp":1648729285968},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1981,1,1]]},"abstract":"<jats:p>Heat denaturation and Pronase degradation of the complex previously formed between benzylpenicillin and the exocellular DD-carboxypeptidase-transpeptidase of Actinomadura R39 yields a heptapeptide H-Leu-Pro-Ala-Ser-Asn-Gly-Val-OH, where the benzylpenicilloyl group is ester-linked to the serine residue. This linkage is very labile and its hydrolysis causes the release of benzylpenicilloate. In contrast, the native benzylpenicilloyl-enzyme complex is very stable (half-life 70 h at 37 degrees C) and its breakdown proceeds via fragmentation of the bound benzylpenicilloyl group [Fuad, Fr\u00e8re, Ghuysen, Duez &amp; Iwatsubo (1976) Biochem. J. 155, 623-629].<\/jats:p>","DOI":"10.1042\/bj1930083","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:26:12Z","timestamp":1439238372000},"page":"83-86","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["The penicillin-binding site in the exocellular <scp>dd<\/scp>-carboxypeptidase-transpeptidase of <i>Actinomadura<\/i> R39"],"prefix":"10.1042","volume":"193","author":[{"given":"C","family":"Duez","sequence":"first","affiliation":[]},{"given":"B","family":"Joris","sequence":"additional","affiliation":[]},{"given":"J M","family":"Fr\u00e8re","sequence":"additional","affiliation":[]},{"given":"J M","family":"Ghuysen","sequence":"additional","affiliation":[]},{"given":"J","family":"Van Beeumen","sequence":"additional","affiliation":[]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/193\/1\/83\/575358\/bj1930083.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/193\/1\/83\/575358\/bj1930083.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T14:43:52Z","timestamp":1637851432000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/193\/1\/83\/19121\/The-penicillin-binding-site-in-the-exocellular-dd"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1981,1,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1930083","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1981,1,1]]}}}