{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T16:04:28Z","timestamp":1775923468491,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1981,2,1]]},"abstract":"<jats:p>The D-ribulokinase and D-xylulokinase of Klebsiella aerogenes were purified to homogeneity from Escherichia coli K12 construct strains that synthesized these enzymes constitutively. The D-ribulokinase, which is encoded in the ribitol operon, is active as a dimer of 60 000 subunit mol.wt., whereas the D-xylulokinase, which is encoded in the D-arabitol operon, is active as a dimer of 54 000 subunit mol.wt. The amino acid compositions and N-terminal sequences of both pentulokinases are reported. The Kapp. values of the enzymes for their D-pentulose substrates were determined, and the D-ribulokinase was shown to have a low-affinity side-specificity for ribitol and D-arabitol. These results are discussed in the context of the evolution of the Klebsiella aerogenes pentitol operons.<\/jats:p>","DOI":"10.1042\/bj1930513","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T20:26:12Z","timestamp":1439238372000},"page":"513-524","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Purification and properties of <scp>d<\/scp>-ribulokinase and <scp>d<\/scp>-xylulokinase from <i>Klebsiella aerogenes<\/i>"],"prefix":"10.1042","volume":"193","author":[{"given":"M S","family":"Neuberger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B S","family":"Hartley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J E","family":"Walker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/193\/2\/513\/575914\/bj1930513.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/193\/2\/513\/575914\/bj1930513.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T14:04:47Z","timestamp":1637849087000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/193\/2\/513\/17302\/Purification-and-properties-of-d-ribulokinase-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,2,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1981,2,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj1930513","relation":{},"ISSN":["0264-6021"],"issn-type":[{"value":"0264-6021","type":"print"}],"subject":[],"published":{"date-parts":[[1981,2,1]]}}}