{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T13:53:41Z","timestamp":1779890021876,"version":"3.53.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,1,1]]},"abstract":"<jats:p>Phosphorylation of the endogenous GSK3\u03b1 (glycogen synthase kinase-3\u03b1) at Tyr279 and GSK3\u03b2 at Tyr216 was suppressed in HEK-293 or SH-SY5Y cells by incubation with pharmacological inhibitors of GSK3, but not by an Src-family inhibitor, 4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine (PP2), or a general protein tyrosine kinase inhibitor (genistein). GSK3\u03b2 transfected into HEK-293 cells or Escherichia coli became phosphorylated at Tyr216, but catalytically inactive mutants did not. GSK3\u03b2 expressed in insect Sf 21 cells or E. coli was extensively phosphorylated at Tyr216, but the few molecules lacking phosphate at this position could autophosphorylate at Tyr216in vitro after incubation with MgATP. The rate of autophosphorylation was unaffected by dilution and was suppressed by the GSK3 inhibitor kenpaullone. Wild-type GSK3\u03b2 was unable to catalyse the tyrosine phosphorylation of catalytically inactive GSK3\u03b2 lacking phosphate at Tyr216. Our results indicate that the tyrosine phosphorylation of GSK3 is an intramolecular autophosphorylation event in the cells that we have studied and that this modification enhances the stability of the enzyme.<\/jats:p>","DOI":"10.1042\/bj20031259","type":"journal-article","created":{"date-parts":[[2004,1,13]],"date-time":"2004-01-13T09:02:27Z","timestamp":1073984547000},"page":"249-255","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":260,"title":["Further evidence that the tyrosine phosphorylation of glycogen synthase kinase-3 (GSK3) in mammalian cells is an autophosphorylation event"],"prefix":"10.1042","volume":"377","author":[{"given":"Adam","family":"COLE","sequence":"first","affiliation":[{"name":"MRC Protein Phosphorylation Unit, School of Life Sciences, MSI\/WTB Complex, Dow Street, Dundee DD1 5EH, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheelagh","family":"FRAME","sequence":"additional","affiliation":[{"name":"Division of Signal Transduction Therapy, School of Life Sciences, MSI\/WTB Complex, Dow Street, Dundee DD1 5EH, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"COHEN","sequence":"additional","affiliation":[{"name":"MRC Protein Phosphorylation Unit, School of Life Sciences, MSI\/WTB Complex, Dow Street, Dundee DD1 5EH, Scotland, U.K."},{"name":"Division of Signal Transduction Therapy, School of Life Sciences, MSI\/WTB Complex, Dow Street, Dundee DD1 5EH, Scotland, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/377\/1\/249\/712631\/bj3770249.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/377\/1\/249\/712631\/bj3770249.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,22]],"date-time":"2021-11-22T13:51:59Z","timestamp":1637589119000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/377\/1\/249\/43171\/Further-evidence-that-the-tyrosine-phosphorylation"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj20031259","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1,1]]}}}