{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:38:44Z","timestamp":1758404324799},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,6,15]]},"abstract":"<jats:p>The effects of insulin and anti-(insulin receptor) monoclonal antibodies on tyrosine phosphorylation were investigated in fibroblasts transfected with human insulin receptor cDNA (NIH 3T3HIR3.5 cells) using anti-phosphotyrosine immunoblotting. Insulin increased levels of tyrosine phosphorylation in two major proteins of molecular mass 97 kDa (pp97, assumed to be the insulin receptor beta-subunit) and 185 kDa (pp185). Insulin-mimetic anti-receptor antibodies also stimulated tyrosine phosphorylation of both pp97 and pp185. The observation of antibody-stimulated pp97 phosphorylation, as detected by immunoblotting, is in contrast with previous data which failed to show receptor autophosphorylation in NIH 3T3HIR3.5 cells labelled with [32P]P1. The effect of insulin on pp97 was maximal within 1 min, but the response to antibody was apparent only after a lag of 1-2 min and rose steadily over 20 min. The absolute level of antibody-stimulated phosphorylation of both pp97 and pp185 after 20 min was only about 20% of the maximum level induced by equivalent concentrations of insulin, even at concentrations of antibody sufficient for full occupancy of receptors. Another insulin-mimetic agent, wheat-germ agglutinin, stimulated receptor autophosphorylation with kinetics similar to those produced by the antibody. It is suggested that the relatively slow responses to both agents may be a function of the dependence on receptor cross-linking. These data are consistent with a role for the insulin receptor tyrosine kinase activity in the mechanism of action of insulin-mimetic anti-receptor antibodies.<\/jats:p>","DOI":"10.1042\/bj2680615","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:16:34Z","timestamp":1439241394000},"page":"615-620","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Anti-(insulin receptor) monoclonal antibody-stimulated tyrosine phosphorylation in cells transfected with human insulin receptor cDNA"],"prefix":"10.1042","volume":"268","author":[{"given":"N P J","family":"Brindle","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QR, U.K."}]},{"given":"J M","family":"Tavare","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, U.K."}]},{"given":"M","family":"Dickens","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Bristol Medical School, University Walk, Bristol BS8 1TD, U.K."}]},{"given":"J","family":"Whittaker","sequence":"additional","affiliation":[{"name":"Division of Endocrinology, Department of Medicine, Health Science Center, State University of New York at Stony Brook, Stony Brook, NY 11794-8154, U.S.A."}]},{"given":"K","family":"Siddle","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QR, U.K."}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/268\/3\/615\/599383\/bj2680615.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/268\/3\/615\/599383\/bj2680615.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:37:38Z","timestamp":1637865458000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/268\/3\/615\/26300\/Anti-insulin-receptor-monoclonal-antibody"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,6,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2680615","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6,15]]}}}