{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T00:18:22Z","timestamp":1704327502258},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,7,1]]},"abstract":"<jats:p>A filter-based immunofluorescence-microscopy method for obtaining microtubule lengths has been developed and evaluated. Kinetic constants and mean lengths obtained show close agreement with those obtained by complementary methods applied to chick brain MAP2-tubulin microtubule protein in NaCl-supplemented buffer. The filter-based method has been used to estimate tubulin subunit flux (Jon) resulting from isothermal dilution of microtubule populations to various free tubulin concentrations, (c). This experimental Jon(c) plot is significantly different from that predicted by a variety of theoretical models, but is consistent with a \u2018lateral cap\u2019 model of dynamic instability [Bayley, Schilstra &amp; Martin (1990) J. Cell. Sci. 95, 33-48] adapted to accommodate the observed vectorial GTP hydrolysis.<\/jats:p>","DOI":"10.1042\/bj2770245","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:22:29Z","timestamp":1439241749000},"page":"245-253","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Assembly of chick brain MAP2-tubulin microtubule protein. Analysis of tubulin subunit flux rates by immunofluorescence microscopy"],"prefix":"10.1042","volume":"277","author":[{"given":"M F","family":"Symmons","sequence":"first","affiliation":[{"name":"Biophysics Section, Blackett Laboratory, Imperial College of Science, Technology and Medicine, London, SW7 2BZ, U.K."}]},{"given":"R G","family":"Burns","sequence":"additional","affiliation":[{"name":"Biophysics Section, Blackett Laboratory, Imperial College of Science, Technology and Medicine, London, SW7 2BZ, U.K."}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/277\/1\/245\/603718\/bj2770245.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/277\/1\/245\/603718\/bj2770245.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T15:21:36Z","timestamp":1637680896000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/277\/1\/245\/27072\/Assembly-of-chick-brain-MAP2-tubulin-microtubule"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2770245","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,7,1]]}}}