{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:58:08Z","timestamp":1758279488638},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,9,1]]},"abstract":"<jats:p>We have shown that platelets stimulated with thrombin or guanosine 5\u2032-[gamma-thio]triphosphate (GTP[S]), both of which activate phospholipase C and protein kinase C (PKC), show enhancement of 3-phosphorylated phosphoinositide accumulation (3-PPI). We now report the following. (1) Inhibition of thrombin- or GTP[S]-stimulated PKC by pseudo-substrate peptide (RFARK) added to permeabilized platelets markedly inhibits 3-PPI, whereas the serine\/threonine phosphatase inhibitor, okadaic acid, promotes 3-PPI. PKC activity, insufficient in itself for fully activating 3-PPI, appears crucial to receptor and post-receptor stimulation of 3-PPI, even when tyrosine phosphorylation is unimpaired. (2) Alteration of Gi by ADP-ribosylation only slightly affects the stimulation of 3-PPI by thrombin, and activation of the G-protein Gi by adrenaline has no effect on 3-PPI. (3) Inhibition of PKC blocks activated secretion of platelet-derived growth factor (PDGF). However, PDGF cannot promote platelet 3-PPI, and thus cannot account for the inhibitory effects of RFARK on 3-PPI.<\/jats:p>","DOI":"10.1042\/bj2780475","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:39:42Z","timestamp":1439501982000},"page":"475-480","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Protein kinase C regulates the stimulated accumulation of 3-phosphorylated phosphoinositides in platelets"],"prefix":"10.1042","volume":"278","author":[{"given":"W G","family":"King","sequence":"first","affiliation":[{"name":"Department of Biochemistry University of Vermont College of Medicine, Burlington, VT 05405, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G L","family":"Kucera","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Vermont College of Medicine, Burlington, VT 05405, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Sorisky","sequence":"additional","affiliation":[{"name":"Metabolic Unit, University of Vermont College of Medicine, Burlington, VT 05405, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Vermont College of Medicine, Burlington, VT 05405, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S E","family":"Rittenhouse","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Vermont College of Medicine, Burlington, VT 05405, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/278\/2\/475\/603891\/bj2780475.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/278\/2\/475\/603891\/bj2780475.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T15:30:19Z","timestamp":1637681419000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/278\/2\/475\/27270\/Protein-kinase-C-regulates-the-stimulated"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2780475","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,9,1]]}}}