{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T14:02:33Z","timestamp":1776866553132,"version":"3.51.2"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,9,15]]},"abstract":"<jats:p>A human hippocampus cDNA library in lambda ZAP II was screened by hybridization with a rat brain inositol 1,4,5-trisphosphate (InsP3) 3-kinase cDNA. Two clones (hh6 and hh3) were isolated and sequenced. The insert of clone hh6 was shown to correspond to the 3\u2032 end of the coding sequence of 50,000-Mr InsP3 3-kinase (referred to as 3-kinase-A). Sequencing of the clone hh3 insert yielded an open reading frame encoding a 472-amino acid protein with a calculated Mr of 53,451 (referred to as 3-kinase-B). The C-terminal part of 3-kinase-B (residues 187-462) was 68% identical with 3-kinase-A in amino acid sequence. The cDNA of clone hh3 was rescued as a Bluescript plasmid and expressed in Escherichia coli as a beta-galactosidase fusion product. It showed InsP3 3-kinase activity that was stimulated in the presence of Ca2+\/calmodulin (more than 7-fold in a crude bacterial lysate from expressed plasmid). Regeneration of InsP3 3-kinase activity after SDS\/PAGE identified a major polypeptide (Mr 60,000-65,000). The Km for InsP3 of expressed 3-kinase-B was 1.6 microM. These data provide molecular evidence for the existence of InsP3 3-kinase isoenzymes.<\/jats:p>","DOI":"10.1042\/bj2780883","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:39:42Z","timestamp":1439501982000},"page":"883-886","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Molecular cloning and expression of a new putative inositol 1,4,5-trisphosphate 3-kinase isoenzyme"],"prefix":"10.1042","volume":"278","author":[{"given":"K","family":"Takazawa","sequence":"first","affiliation":[{"name":"Institut de Recherche Interdisciplinaire (IRIBHN), Universit\u00e9 Libre de Bruxelles, Campus Erasme, 808 route de Lennik, B-1070 Brussels, Belgium"}]},{"given":"J","family":"Perret","sequence":"additional","affiliation":[{"name":"Institut de Recherche Interdisciplinaire (IRIBHN), Universit\u00e9 Libre de Bruxelles, Campus Erasme, 808 route de Lennik, B-1070 Brussels, Belgium"}]},{"given":"J E","family":"Dumont","sequence":"additional","affiliation":[{"name":"Institut de Recherche Interdisciplinaire (IRIBHN), Universit\u00e9 Libre de Bruxelles, Campus Erasme, 808 route de Lennik, B-1070 Brussels, Belgium"}]},{"given":"C","family":"Erneux","sequence":"additional","affiliation":[{"name":"Institut de Recherche Interdisciplinaire (IRIBHN), Universit\u00e9 Libre de Bruxelles, Campus Erasme, 808 route de Lennik, B-1070 Brussels, Belgium"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/278\/3\/883\/604038\/bj2780883.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/278\/3\/883\/604038\/bj2780883.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T15:31:05Z","timestamp":1637681465000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/278\/3\/883\/27291\/Molecular-cloning-and-expression-of-a-new-putative"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,9,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2780883","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,9,15]]}}}