{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T11:50:57Z","timestamp":1770292257459,"version":"3.49.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,6,1]]},"abstract":"<jats:p>The expression of cell-surface peptidases was examined in two human colon carcinoma cell lines, Caco-2 and HT-29. Enzymic assays revealed the presence of eight cell-surface peptidases on a Caco-2 cell line (passage number 82-88), namely aminopeptidase N, dipeptidyl peptidase IV, peptidyl dipeptidase A (angiotension-converting enzyme), aminopeptidase P, aminopeptidase W, endopeptidase-24.11, gamma-glutamyl transpeptidase and membrane dipeptidase. The presence of dipeptidyl peptidase IV and endopeptidase-24.11 was also confirmed immunochemically. After 15 days culture, the activities of aminopeptidase P, peptidyl dipeptidase A and alkaline phosphatase activities on Caco-2 cells reached a plateau, and that of membrane dipeptidase began to decline. In contrast, aminopeptidase N, dipeptidyl peptidase IV and endopeptidase-24.11 activities were still rising after 26 days in culture. Caco-2 cells of passage number 181-183 were found to lack endopeptidase-24.11, but maintained dipeptidyl peptidase IV expression. Two populations of HT-29 cells were surveyed. Both the standard, undifferentiated population and a differentiated population expressed only three peptidases: dipeptidyl peptidase IV, aminopeptidase W and carboxypeptidase M. In the differentiated HT-29 cells the activity of dipeptidyl peptidase IV after 14-21 days was beginning to plateau whereas aminopeptidase W activity was still rising and that of carboxypeptidase M had begun to decline. These differences in activity profiles observed among this group of cell-surface peptidases indicate that these cell lines, especially Caco-2, are useful models to study the regulation of their expression.<\/jats:p>","DOI":"10.1042\/bj2840595","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:20:57Z","timestamp":1439500857000},"page":"595-601","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":139,"title":["A survey of membrane peptidases in two human colonic cell lines, Caco-2 and HT-29"],"prefix":"10.1042","volume":"284","author":[{"given":"S","family":"Howell","sequence":"first","affiliation":[{"name":"Membrane Peptidase Research Group, Department of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A J","family":"Kenny","sequence":"additional","affiliation":[{"name":"Membrane Peptidase Research Group, Department of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A J","family":"Turner","sequence":"additional","affiliation":[{"name":"Membrane Peptidase Research Group, Department of Biochemistry and Molecular Biology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/284\/2\/595\/607010\/bj2840595.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/284\/2\/595\/607010\/bj2840595.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T21:56:48Z","timestamp":1637704608000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/284\/2\/595\/28149\/A-survey-of-membrane-peptidases-in-two-human"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,6,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2840595","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,6,1]]}}}