{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T15:27:20Z","timestamp":1760369240097},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,7,1]]},"abstract":"<jats:p>A subtractive hybridization and cloning strategy was used to identify genes that are up-regulated in regenerating compared with normal skeletal muscle. The gastrocnemius muscle of CD1 mice was injected with a myotoxic agent (BaCl2). A cDNA library was constructed from the regenerating muscle, and was screened with subtracted probes enriched in genes up-regulated during regeneration. Cofilin and vimentin cDNA clones were isolated. Both cofilin and vimentin were demonstrated to be overexpressed in regenerating compared with non-regenerating muscle (17-fold and 19-fold induction respectively). Cofilin and vimentin mRNAs also exhibited an increased expression in C2C12 myoblasts and a decreased expression in differentiated myotubes. Analysis of the regeneration-induced vimentin enhancer\/promoter region revealed a consensus binding site for the myocyte enhancer factor 2 (MEF2) transcription factors. Electrophoretic mobility-shift assays and in vivo reporter assays revealed that MEF2 DNA-binding activity and transcriptional activation are increased in regenerating skeletal muscle, indicating that they may play a role in the activation of muscle genes during regeneration. These data suggest that both cofilin (an actin-regulatory protein) and vimentin (an intermediate filament) may be key components of the cytoskeletal reorganization that mediates muscle cell development and adult skeletal-muscle repair.<\/jats:p>","DOI":"10.1042\/bj3250087","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:55:16Z","timestamp":1439243716000},"page":"87-93","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Molecular cloning of up-regulated cytoskeletal genes from regenerating skeletal muscle: potential role of myocyte enhancer factor 2 proteins in the activation of muscle-regeneration-associated genes"],"prefix":"10.1042","volume":"325","author":[{"given":"Waleed M.","family":"AKKILA","sequence":"first","affiliation":[{"name":"Departments of Kinesiology and Biology, Faculty of Pure and Applied Science, York University, Toronto, Ontario, Canada M3J 1P3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca L.","family":"CHAMBERS","sequence":"additional","affiliation":[{"name":"Departments of Kinesiology and Biology, Faculty of Pure and Applied Science, York University, Toronto, Ontario, Canada M3J 1P3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga I.","family":"ORNATSKY","sequence":"additional","affiliation":[{"name":"Departments of Kinesiology and Biology, Faculty of Pure and Applied Science, York University, Toronto, Ontario, Canada M3J 1P3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"McDERMOTT","sequence":"additional","affiliation":[{"name":"Departments of Kinesiology and Biology, Faculty of Pure and Applied Science, York University, Toronto, Ontario, Canada M3J 1P3"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/325\/1\/87\/624412\/bj3250087.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/325\/1\/87\/624412\/bj3250087.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:29:53Z","timestamp":1637713793000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/325\/1\/87\/33804\/Molecular-cloning-of-up-regulated-cytoskeletal"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3250087","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,7,1]]}}}