{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T14:31:38Z","timestamp":1776954698969,"version":"3.51.4"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,9,1]]},"abstract":"<jats:p>Complement factor H (FH) and factor-H-like protein 1 (FHL-1) are human plasma proteins with regulatory functions in the alternative pathway of complement activation. FH and FHL-1 are organized in repetitive elements termed short consensus repeats (SCRs) and the seven SCRs of FHL-1 are identical with the N-terminal domain of the 20 SCRs of FH. The fourth SCR of both proteins (SCR 4) includes the sequence Arg-Gly-Asp (RGD), a motif that is responsible for the major adhesive activity of matrix proteins like fibronectin. A synthetic hexapeptide with the sequence ERGDAV derived from the RGD domain of FH\/FHL-1 interferes with cell attachment to a fibronectin matrix. Although the identical motif is present in both FH and FHL-1, only FHL-1 acts as a matrix for cell spreading and attachment, thus the two proteins differ in function. The adhesive activity of FHL-1 is localized to the RGD-containing SCR 4 by the use of recombinant fragments. All three analysed anchorage-dependent cell lines (CCl64, C32 and MRC-5) adhere to an FHL-1 matrix. The use of synthetic peptides in competition assays, on either FHL-1-derived or fibronectin matrices, shows that the cellular receptors binding to the FH\/FHL-1-derived RGD motif are related to or identical with integrin receptors which interact with fibronectin. The identification of a functional adhesive domain in the FH\/FHL-1 sequence demonstrates, at least for FHL-1, a role in cell attachment and adhesion.<\/jats:p>","DOI":"10.1042\/bj3260321","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:55:50Z","timestamp":1439243750000},"page":"321-327","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["The human complement regulatory factor-H-like protein 1, which represents a truncated form of factor H, displays cell-attachment activity"],"prefix":"10.1042","volume":"326","author":[{"given":"Jens","family":"HELLWAGE","sequence":"first","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"K\u00dcHN","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter F.","family":"ZIPFEL","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, Bernhard-Nocht-Strasse 74, D-20359 Hamburg, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/326\/2\/321\/625589\/bj3260321.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/326\/2\/321\/625589\/bj3260321.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:23:11Z","timestamp":1637713391000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/326\/2\/321\/33267\/The-human-complement-regulatory-factor-H-like"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3260321","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9,1]]}}}